Units listed are MBU (molecular biology units).
Storage Buffer: 10mM Tris (pH 7.5 @ 25°C), 100mM NaCl, 50% glycerol.
QC Tests: Activity, SDS-PAGE/purity, endonuclease, DNase, RNase, blue/white cloning assay.
Unit Definition: One MBU is defined as the amount of enzyme required to dephosphorylate 1μg of pGEM® DNA with 3´ overhangs in 15 minutes at 37°C in a 50μl reaction using any Promega restriction enzyme buffer (except F). Dephosphorylation is defined as preventing >95% of the plasmid from ligating (analyzed by agarose gel electrophoresis).