MyGlo Instrument now $4950. Use code GETMYGLO at checkout to get 10% off MyGlo. Shop Now ›

RQ1 RNase-Free DNase

M6101_RQ1-RNase-Free-DNase--1-000u_3
customize-this-small
View information on global supply logistics

Degrades Single-Stranded or Double-Stranded DNA

  • Produces 3´ hydroxyl oligonucleotides during degradation
  • Preparation qualified for use in applications where maintaining the integrity of RNA is critical
  • Supplied at a concentration of 1u/μl

Size

Catalog number selected: M6101

$ 61.00
Your price:
Add to Cart
Product Discontinued
Add to Helix
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
RQ1 RNase-Free DNase
1,000u
$ 61.00
Your price: Log in

RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. The enzyme is provided with 10X Reaction Buffer (400mM Tris-HCl [pH 8.0 at 25°C], 100mM MgSO4, 10mM CaCl2) and Stop Buffer (20mM EGTA [pH 8.0 at 25°C])

Applications

  • Preparation of DNA-free RNA.
  • Degradation of DNA from RNA transcription systems.
  • Nick translation of DNA.
  • Studying DNA:protein interactions by DNase I footprinting.

Storage Buffer: 10mM HEPES (pH 7.5 at 25°C), 50% (v/v) glycerol, 10mM CaCl2, 10mM MgCl2.

Source: Bovine pancreas.

QC Tests: Activity, RNase.

Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1μg of DNA in 10 minutes at 37°C in 50μl of a buffer containing 40mM Tris-HCl (pH 7.9 at 25°C), 10mM NaCl, 6mM MgCl2, 10mM CaCl2.

Specifications

Whats in the box

Item Part # Size

RQ1 DNase 10X Reaction Buffer

M198A 1 × 1ml

RQ1 DNase Stop Solution

M199A 1 × 1ml

RQ1 RNase-Free DNase

M610A 1 × 1,000u

Certificate of Analysis

Search by lot number

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-stephen

Stephen

UK