The Reporter Vector (RV) Sequencing Primers are designed for use with the pGL3 and pGL4 Luciferase Vectors, Chroma-Luc™ Vectors and pCAT™3 Reporter Vectors. RVprimer3 binds upstream of the luc+, luc2 or CAT gene, and sequencing runs clockwise across the multiple cloning region. RVprimer4 binds downstream of the luc+, luc2 or CAT polyadenylation region in the Promoter and Basic Vectors and downstream of the SV40 enhancer region of the Enhancer and Control Vectors. The primers are supplied dried.
- RVprimer3: 5´-d(CTAGCAAAATAGGCTGTCCC)-3´
- RVprimer4: 5´-d(GACGATAGTCATGCCCCGCG)-3´
- Determination of the exact position of generated deletions.
- Confirmation of production of a site-specific mutation.