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Mung Bean Nuclease

M4311_Mung-Bean-Nuclease--2-000u_3
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Endonuclease that Degrades ssDNA and RNA to Yield 5´ Phosphoryl-Terminated Products

  • Removes protruding single-stranded termini in double-stranded DNA
  • Selectively cleaves single-stranded nucleic acid, e.g., mRNA mapping experiments
  • Supplied at a concentration of 50–100u/μl

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Catalog number selected: M4311

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Mung Bean Nuclease
2,000u
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Mung Bean Nuclease catalyzes the degradation of single-stranded DNA and RNA endonucleolytically to yield 5´ phosphoryl-terminated products. While the nuclease prefers ssDNA over dsDNA by 30,000-fold, at very high concentrations the enzyme degrades double-stranded DNA from both ends. Mung Bean Nuclease has been used for transcript mapping studies, for flushing staggered ends and for the separation of cDNA strands after synthesis with reverse transcriptase and DNA Polymerase I. Mung Bean Nuclease is provided with 10X Reaction Buffer: 300mM sodium acetate (pH 5.0 at 15°C), 500mM NaCl, 10mM ZnCl2.

References

  1. Ardelt, W. and Laskowski, M., Sr. (1971) Biochem. Biophys. Res. Comm. 44, 1205–11.
  2. Kroeker, W.D. et al. (1976) Biochemistry 15, 4463–7.
  3. Mathis, D.J. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 7383–7.
  4. Green, M.R. and Roeder, R.G. (1980) Cell 22, 231–42.
  5. Gubler, U. (1987) Meth. Enzymol. 152, 330–5.

Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM NaCl, 0.01% Triton® X-100 and 50% glycerol.

Source: Mung bean sprouts.

QC Tests: Activity, endonuclease.

Unit Definition: One unit is defined as the amount of enzyme required to produce 1μg of acid-soluble nucleotides per minute at 37°C in 30mM sodium acetate (pH 5.0), 50mM NaCl, 1mM ZnCl2, 0.5mg/ml denatured calf thymus DNA and 5% glycerol.

Specifications

What's in the box?

Item Part # Size

Mung Bean Nuclease

M431A 1 × 2,000u

Mung Bean Nuclease 10X Reaction Buffer

M432A 1 × 1ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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