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DNA 5´ End-Labeling System

U2010_DNA-5--End-Labeling-Sys-_3

Phosphorylates RNA, ssDNA and dsDNA Ends with a Purchased Radiolabeled Nucleotide

  • System includes enzymes, buffers and control DNA standards for measuring reaction efficiencies (except radionucleotides)
  • Flexible labeling works with [γ-32P]ATP, [γ-33P]ATP or [γ-35S]ATP

Size

Catalog number selected: U2010

$ 128.00
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DNA 5´ End-Labeling System
10 reactions
$ 128.00
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The DNA 5´ End-Labeling System is a complete system for phosphorylating both double- and single-stranded DNA and RNA with T4 Polynucleotide Kinase and [γ-32P]ATP. Just supply the radionucleotide. Calf Intestinal Alkaline Phosphatase is included for removal of the 5´-phosphate prior to labeling with T4 Polynucleotide Kinase.

Applications

  • Preparing hybridization probes.
  • Preparing sequencing primers.
  • Labeling marker DNAs and RNAs for autoradiography.
  • Preparing probes for gel shift assays.

Specifications

What's in the box?

Item Part # SizeAvailable Separately

pGEM® DNA Markers

G174B 1 × 10μg

Alkaline Phosphatase, Calf Intestinal

M182B 1 × 100u

Alkaline Phosphatase 10X Buffer

M183A 1 × 500μl View Product

T4 Polynucleotide Kinase

M410A 1 × 100u View Product

T4 PNK 10X Buffer

Q561C 1 × 500μl

Lambda gt10 Reverse Primer

U204A 1 × 500ng

SDS

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Download SDSPDF (199 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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