pH6HTC His6HaloTagŪ T7 Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pH6HTC His6HaloTagŪ T7 Vector sequence reference points: Base pairs 3984 T7 RNA polymerase promoter (-17 to +3) 21-40 Multiple cloning region 61-129 HaloTagŪ linker region 124-168 TEV protease recognition region 139-159 HaloTagŪ region 169-1059 His6HaloTagŪ region 169-1077 His6 region 1060-1077 T7 terminator 1102-1149 Beta-lactamase (AmpR) coding region 1483-2343 ColE1-derived plasmid origin of replication 2498-2534 rrnB transcription terminator 3541-3942 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG TATTTTAACT 51 TTACTAAGGA GCGATCGCTT CCGAATTCCT ACCGCGGATA TCTAGATTTG 101 GGCCCAATTC CTGCAGGCGA GCTCTCGAGC CAACCACTGA GGATCTGTAC 151 TTTCAGAGCG ATAACGATGG ATCCGAAATC GGTACTGGCT TTCCATTCGA 201 CCCCCATTAT GTGGAAGTCC TGGGCGAGCG CATGCACTAC GTCGATGTTG 251 GTCCGCGCGA TGGCACCCCT GTGCTGTTCC TGCACGGTAA CCCGACCTCC 301 TCCTACGTGT GGCGCAACAT CATCCCGCAT GTTGCACCGA CCCATCGCTG 351 CATTGCTCCA GACCTGATCG GTATGGGCAA ATCCGACAAA CCAGACCTGG 401 GTTATTTCTT CGACGACCAC GTCCGCTTCA TGGATGCCTT CATCGAAGCC 451 CTGGGTCTGG AAGAGGTCGT CCTGGTCATT CACGACTGGG GCTCCGCTCT 501 GGGTTTCCAC TGGGCCAAGC GCAATCCAGA GCGCGTCAAA GGTATTGCAT 551 TTATGGAGTT CATCCGCCCT ATCCCGACCT GGGACGAATG GCCAGAATTT 601 GCCCGCGAGA CCTTCCAGGC CTTCCGCACC ACCGACGTCG GCCGCAAGCT 651 GATCATCGAT CAGAACGTTT TTATCGAGGG TACGCTGCCG ATGGGTGTCG 701 TCCGCCCGCT GACTGAAGTC GAGATGGACC ATTACCGCGA GCCGTTCCTG 751 AATCCTGTTG ACCGCGAGCC ACTGTGGCGC TTCCCAAACG AGCTGCCAAT 801 CGCCGGTGAG CCAGCGAACA TCGTCGCGCT GGTCGAAGAA TACATGGACT 851 GGCTGCACCA GTCCCCTGTC CCGAAGCTGC TGTTCTGGGG CACCCCAGGC 901 GTTCTGATCC CACCGGCCGA AGCCGCTCGC CTGGCCAAAA GCCTGCCTAA 951 CTGCAAGGCT GTGGACATCG GCCCGGGTCT GAATCTGCTG CAAGAAGACA 1001 ACCCGGACCT GATCGGCAGC GAGATCGCGC GCTGGCTGTC TACTCTGGAG 1051 ATTTCCGGTC ATCATCACCA TCACCACTAA TAGTCAACCG CTGAGCAATA 1101 ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC 1151 TGAAAGGAGG AACTATATCC GGTTCGCTTG CTGTCCATAA AACCGCCCAG 1201 TCTAGCTATC GCCATGTAAG CCCACTGCAA GCTACCTGCT TTCTCTTTGC 1251 GCTTGCGTTT TCCCTTGTCC AGATAGCCCA GTAGCTGACA TTCATCCGGG 1301 GTCAGCACCG TTTCTGCGGA CTGGCTTTCT ACGTAATGGT TTCTTAGACG 1351 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT 1401 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT 1451 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC 1501 CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC 1551 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG 1601 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 1651 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TCAAAGTTCT 1701 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG 1751 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC 1801 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC 1851 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACTA 1901 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT 1951 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA 2001 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA 2051 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA 2101 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT 2151 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT 2201 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC 2251 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG 2301 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAATTCGAAA 2351 TGACCGACCA AGCGACGCCC AACCGGTATC AGCTCACTCA AAGGCGGTAA 2401 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA 2451 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 2501 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 2551 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 2601 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 2651 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 2701 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC 2751 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 2801 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 2851 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 2901 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT 2951 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 3001 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT 3051 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATTTCAAG AAGATCCTTT 3101 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 3151 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 3201 TAGTCCGGAA ATACAGGAAC GCACGCTGGA TGGCCCTTCG CTGGGATGGT 3251 GAAACCATGA AAAATGGCAG CTTCAGTGGA TTAAGTGGGG GTAATGTGGC 3301 CTGTACCCTC TGGTTGCATA GGTATTCATA CGGTTAAAAT TTATCAGGCG 3351 CGATTGCGGC AGTTTTTCGG GTGGTTTGTT GCCATTTTTA CCTGTCTGCT 3401 GCCGTGATCG CGCTGAACGC GTTTTAGCGG TGCGTACAAT TAAGGGATTA 3451 TGGTAAATCC ACTTACTGTC TGCCCTCGTA GCCATCGAGA TAAACCGCAG 3501 TACTCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TTTTCAGCCT 3551 GATACAGATT AAATCAGAAC GCAGAAGCGG TCTGATAAAA CAGAATTTGC 3601 CTGGCGGCAG TAGCGCGGTG GTCCCACCTG ACCCCATGCC GAACTCAGAA 3651 GTGAAACGCC GTAGCGCCGA TGGTAGTGTG GGGTCTCCCC ATGCGAGAGT 3701 AGGGAACTGC CAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG 3751 GCCTTTCGTT TTATCTGTTG TTTGTCGGTG AACGCTCTCC TGAGTAGGAC 3801 AAATCCGCCG GGAGCGGATT TGAACGTTGC GAAGCAACGG CCCGGAGGGT 3851 GGCGGGCAGG ACGCCCGCCA TAAACTGCCA GGCATCAAAT TAAGCAGAAG 3901 GCCATCCTGA CGGATGGCCT TTTTGCGTTT CTACAAACTC TTTTGTTTAT 3951 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCAT Sequence file prepared 02-November-2011.