We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

United States
Use code TVECTOR20 at checkout to get 20% off pGEM®-T Vector Systems! Show Now >

pGEM®-9Zf(–) Vector

P2391_pGEM--9Zf----Vector--20ug_3
Click here to customize this product

Versatile Cloning Vector That Can Efficiently Synthesize RNA In vitro and Produce ssDNA

  • Insert can be excised from vector with the SP6 and T7 RNA polymerase promoters
  • Blue/white screening to identify recombinant clones
  • Multiple cloning site provides a convenient selection of restriction sites for cloning

Size

Catalog number selected: P2391

$ 135.00
Your price:
Add to Cart
This product is discontinued
pGEM®-9Zf(–) Vector
20μg
$ 135.00
Your price: Log in

The pGEM®-9Zf(–) Vector is a recombinant plasmid designed to provide a versatile range of cloning strategies, and contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide identifies recombinant clones by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for NsiI, SpeI, HindIII, XbaI, EcoRI, SalI and SacI.

0288VA05_4A-W

pGEM®-9Zf(–) Vector GenBank® Accession Number X65312 and vector sequence text file.

Protocols

Complete Protocol

Specifications

What's in the box?

Item Part # Size

pGEM®-9Zf(–) Vector

 P239A 1 × 20μg

SDS

Choose language:

Download SDSPDF (67 KB) – English (United States)

Certificate of Analysis

See all certificates for P2391

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Resources

Citations

Similar Products

pGEM-3Zf(+/–) Vectors

Standard cloning vectors that can produce ssDNA and in vitro transcribe sense and antisense RNA; offers blue/white screening.

P2271, P2261

pGEM-5Zf(+) Vector

A standard cloning vector used as a template for in vitro transcription or for production of circular ssDNA.

P2241

pGEM-7Zf(+/–) Vectors

Standard cloning vectors used as templates for in vitro transcription or production of circular ssDNA.

P2251, P2371

pGEM®-11Zf(+) Vector

A standard cloning vector that can be used for in vitro transcription and produce ssDNA; has blue/white screening.

P2411

Frequently Used With

JM109 Competent Cells

E. coli strain ready for transformation; offers blue/white selection.

L2005, L1001, L2001

RiboMAX™ Large-Scale RNA Production Systems

Generates capped or uncapped RNA transcripts for in vitro or in vivo translation.

P1280, P1300

Riboprobe® Systems

Generate high-specific-activity RNA probes or transcripts; choice of SP6, T7 or T3 systems.

P1420, P1430, P1440

Riboprobe® Combination Systems

Generate high-specific-activity RNA probes or transcripts; choice of SP6/T7 or T3/T7 systems.

P1450, P1460

Let's find the product that meets your needs.

Talk to a Scientist

Sylvia

Silvia

Italy
Choose your country

Americas

Brazil
/
Canada
/
United States

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
Estonia
Finland
France
/
Germany
/
Iceland
Italy
/
Luxembourg
Netherlands
Norway
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom