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pGEM®-9Zf(–) Vector

P2391_pGEM--9Zf----Vector--20ug_3
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Versatile Cloning Vector That Can Efficiently Synthesize RNA In vitro and Produce ssDNA

  • Insert can be excised from vector with the SP6 and T7 RNA polymerase promoters
  • Blue/white screening to identify recombinant clones
  • Multiple cloning site provides a convenient selection of restriction sites for cloning

Size

Catalog number selected: P2391

$ 138.00
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This product is discontinued
pGEM®-9Zf(–) Vector
20μg
$ 138.00
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The pGEM®-9Zf(–) Vector is a recombinant plasmid designed to provide a versatile range of cloning strategies, and contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide identifies recombinant clones by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for NsiI, SpeI, HindIII, XbaI, EcoRI, SalI and SacI.

0288VA05_4A-W

pGEM®-9Zf(–) Vector GenBank® Accession Number X65312 and vector sequence text file.

Protocols

Complete Protocol

Specifications

What's in the box?

Item Part # Size

pGEM®-9Zf(–) Vector

 P239A 1 × 20μg

SDS

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Download SDSPDF (217 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Resources

Citations

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Frequently Used With

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E. coli strain ready for transformation; offers blue/white selection.

L2005, L1001, L2001

RiboMAX™ Large-Scale RNA Production Systems

Compatible with modified or unmodified nucleotides. Produces 2–5mg/ml RNA.

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Riboprobe® Systems

Generate high-specific-activity RNA probes or transcripts; choice of SP6, T7 or T3 systems.

P1420, P1430, P1440

Riboprobe® Combination Systems

Generate high-specific-activity RNA probes or transcripts; choice of SP6/T7 or T3/T7 systems.

P1450, P1460

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Joliene

Joliene

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