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pGEM®-9Zf(–) Vector

Versatile Cloning Vector That Can Efficiently Synthesize RNA In vitro and Produce ssDNA

  • Insert can be excised from vector with the SP6 and T7 RNA polymerase promoters
  • Blue/white screening to identify recombinant clones
  • Multiple cloning site provides a convenient selection of restriction sites for cloning

Size

Catalog number selected: P2391

$ 131.00 Your price: Log In

pGEM®-9Zf(–) Vector
20μg
$ 131.00
Your price: Log In

The pGEM®-9Zf(–) Vector is a recombinant plasmid designed to provide a versatile range of cloning strategies, and contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide identifies recombinant clones by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for NsiI, SpeI, HindIII, XbaI, EcoRI, SalI and SacI.

0288VA05_4A-W

pGEM®-9Zf(–) Vector GenBank® Accession Number X65312 and vector sequence text file.

Protocols

Specifications

What's in the box?

Item Part # SizeAvailable Separately

pGEM®-9Zf(–) Vector

P239A 1 x 20μg

Bacterial Strain JM109

P975A 1 x 500μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for storage recommendations.

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