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pGEM®-3Zf(+/–) Vectors

P2261

Standard Cloning Vectors That Can Produce ssDNA and In vitro Transcribe Sense and Antisense RNA

  • Blue/white screening for easy identification of recombinant clones
  • In vitro transcription using the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
  • The difference between + and – vectors is orientation of the f1 origin

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Size

Catalog number selected: P2271

$ 103.00 Your price: Log In

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pGEM®-3Zf(+/–) Vectors
pGEM®-3Zf(+) Vector/20µg
$ 103.00
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The pGEM®-3Zf(+) and pGEM®-3Zf(–) Vectors are derived from the pGEM®-3Z Vector and contain the origin of replication of the filamentous phage f1. These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII. The pGEM®-3Zf(+) and -3Zf(–) Vectors are identical except for the orientation of the f1 origin and can be used as standard cloning vectors, as templates for in vitro transcription and for the production of circular ssDNA.

Reference

  1. Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
0282VC05_4A-W

pGEM®-3Zf(+) Vector GenBank® Accession Number X65306 and vector sequence text file.

0282VB05_4A-W

pGEM®-3Zf(–) Vector GenBank® Accession Number X65307 and vector sequence text file.

Protocols

Specifications

You are viewing: 20μg Change Configuration

What's in the box?

Item Part # Size

pGEM®-3Zf(+) Vector

P227A 1 x 20μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Specifications

You are viewing: 20μg Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

pGEM®-3Zf(–) Vector

P226A 1 x 20μg

Bacterial Strain JM109

P975A 1 x 500μl View Product

SDS

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Certificate of Analysis

Search for Specific Certificate:

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for storage recommendations.

Resources

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