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pGEM®-11Zf(+) Vector

Standard Cloning Vector Used as a Template for In vitro Transcription and ssDNA Production

  • Blue/white screening for easy identification of recombinant clones
  • In vitro transcription from SP6 and T7 RNA polymerase promoters that flank the multiple cloning region
  • Multiple cloning region provides a convenient selection of restriction sites for cloning

Size

Catalog number selected: P2411

$ 134.00
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This product is discontinued
pGEM®-11Zf(+) Vector
20μg
$ 134.00
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The pGEM®-11Zf(+) Vector is a standard cloning vector that contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide identifies recombinant clones by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for SfiI, SacI, EcoRI, SalI, XhoI, BamHI, ApaI, XbaI, NotI, SphI, NsiI and HindIII. The f(+) of the pGEM®-11Zf(+) Vector refers to the orientation of the f1 origin.

Reference

  1. Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
0290VC05_4A-W

pGEM®-11Zf(+) Vector  GenBank® Accession Number X65313 and vector sequence text file.

Specifications

What's in the box?

Item Part # Size

pGEM®-11Zf(+) Vector

P241A 1 × 20μg

SDS

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Download SDSPDF (67 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

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