pFC30K His6HaloTag® T7 Flexi® Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC30K His6HaloTag® T7 Flexi® Vector sequence reference points: Base pairs 4307 T7 RNA polymerase promoter (-17 to +3) 21-40 SgfI region 61-68 EcoICRI region 447-452 HaloTag® linker region 452-496 TEV protease region 467-487 HaloTag® region 497-1387 His6HaloTag® coding region 497-1405 His6 region 1388-1405 T7 terminator region 1430-1477 Kanamycin resistance(KanR) coding region 1858-2652 ColE1-derived plasmid origin of replication 2821-2857 rrnB transcription terminator 3864-4265 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG TATTTTAACT 51 TTACTAAGGA GCGATCGCCA TGGAATAAGT AAGGAATCCA CATGGCACAG 101 GTTATCAACA CGTTTGACGG GGTTGCGGAT TATCTTCAGA CATATCATAA 151 GCTACCTGAT AATTACATTA CAAAATCAGA AGCACAAGCC CTCGGCTGGG 201 TGGCATCAAA AGGGAACCTT GCAGACGTCG CTCCGGGGAA AAGCATCGGC 251 GGAGACATCT TCTCAAACAG GGAAGGCAAA CTCCCGGGCA AAAGCGGACG 301 AACATGGCGT GAAGCGGATA TTAACTATAC ATCAGGCTTC AGAAATTCAG 351 ACCGGATTCT TTACTCAAGC GACTGGCTGA TTTACAAAAC AACGGACCAT 401 TATCAGACCT TTACAAAAAT CAGATAATGT TTAATGACCC CGTGTCGAGC 451 TCTCGAGCCA ACCACTGAGG ATCTGTACTT TCAGAGCGAT AACGATGGAT 501 CCGAAATCGG TACTGGCTTT CCATTCGACC CCCATTATGT GGAAGTCCTG 551 GGCGAGCGCA TGCACTACGT CGATGTTGGT CCGCGCGATG GCACCCCTGT 601 GCTGTTCCTG CACGGTAACC CGACCTCCTC CTACGTGTGG CGCAACATCA 651 TCCCGCATGT TGCACCGACC CATCGCTGCA TTGCTCCAGA CCTGATCGGT 701 ATGGGCAAAT CCGACAAACC AGACCTGGGT TATTTCTTCG ACGACCACGT 751 CCGCTTCATG GATGCCTTCA TCGAAGCCCT GGGTCTGGAA GAGGTCGTCC 801 TGGTCATTCA CGACTGGGGC TCCGCTCTGG GTTTCCACTG GGCCAAGCGC 851 AATCCAGAGC GCGTCAAAGG TATTGCATTT ATGGAGTTCA TCCGCCCTAT 901 CCCGACCTGG GACGAATGGC CAGAATTTGC CCGCGAGACC TTCCAGGCCT 951 TCCGCACCAC CGACGTCGGC CGCAAGCTGA TCATCGATCA GAACGTTTTT 1001 ATCGAGGGTA CGCTGCCGAT GGGTGTCGTC CGCCCGCTGA CTGAAGTCGA 1051 GATGGACCAT TACCGCGAGC CGTTCCTGAA TCCTGTTGAC CGCGAGCCAC 1101 TGTGGCGCTT CCCAAACGAG CTGCCAATCG CCGGTGAGCC AGCGAACATC 1151 GTCGCGCTGG TCGAAGAATA CATGGACTGG CTGCACCAGT CCCCTGTCCC 1201 GAAGCTGCTG TTCTGGGGCA CCCCAGGCGT TCTGATCCCA CCGGCCGAAG 1251 CCGCTCGCCT GGCCAAAAGC CTGCCTAACT GCAAGGCTGT GGACATCGGC 1301 CCGGGTCTGA ATCTGCTGCA AGAAGACAAC CCGGACCTGA TCGGCAGCGA 1351 GATCGCGCGC TGGCTGTCTA CTCTGGAGAT TTCCGGTCAT CATCACCATC 1401 ACCACTAATA GTCAACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC 1451 TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG 1501 TTCGCTTGCT GTCCATAAAA CCGCCCAGTC TAGCTATCGC CATGTAAGCC 1551 CACTGCAAGC TACCTGCTTT CTCTTTGCGC TTGCGTTTTC CCTTGTCCAG 1601 ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT 1651 GGCTTTCTAC GTGTTCCGCT TCCTTTAGCA GCCCTTGCGC CCTGAGTGCT 1701 TGCGGCAGCG TGAGCTTCAA AAGAATTGCC AGCTGGGGCG CCCTCTGGTA 1751 AGGTTGGGAA GCCCTGCAAA GTAAACTGGA TGGCTTTCTT GCCGCCAAGG 1801 ATCTGATGGC GCAGGGGATC AAGATCTGAT CAAGAGACAG GATGACGGTC 1851 GTTTCGCATG CTTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT 1901 GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 1951 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT 2001 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG 2051 CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 2101 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 2151 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA 2201 TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 2251 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GCACTCGGAT 2301 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC 2351 TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGTAT GCCGGATGGT 2401 GAGGATCTCG TCGTGACTCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 2451 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG 2501 CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 2551 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC 2601 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT 2651 GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCGGT 2701 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 2751 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT 2801 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 2851 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 2901 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 2951 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 3001 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 3051 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC 3101 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 3151 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 3201 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 3251 GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 3301 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 3351 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA 3401 AAAGGATTTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 3451 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 3501 GGATCTTCAC CTAGATCCTT TTATAGTCCG GAAATACAGG AACGCACGCT 3551 GGATGGCCCT TCGCTGGGAT GGTGAAACCA TGAAAAATGG CAGCTTCAGT 3601 GGATTAAGTG GGGGTAATGT GGCCTGTACC CTCTGGTTGC ATAGGTATTC 3651 ATACGGTTAA AATTTATCAG GCGCGATTGC GGCAGTTTTT CGGGTGGTTT 3701 GTTGCCATTT TTACCTGTCT GCTGCCGTGA TCGCGCTGAA CGCGTTTTAG 3751 CGGTGCGTAC AATTAAGGGA TTATGGTAAA TCCACTTACT GTCTGCCCTC 3801 GTAGCCATCG AGATAAACCG CAGTACTCCG GCCACGATGC GTCCGGCGTA 3851 GAGGATCGAG ATCTTTTCAG CCTGATACAG ATTAAATCAG AACGCAGAAG 3901 CGGTCTGATA AAACAGAATT TGCCTGGCGG CAGTAGCGCG GTGGTCCCAC 3951 CTGACCCCAT GCCGAACTCA GAAGTGAAAC GCCGTAGCGC CGATGGTAGT 4001 GTGGGGTCTC CCCATGCGAG AGTAGGGAAC TGCCAGGCAT CAAATAAAAC 4051 GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG 4101 GTGAACGCTC TCCTGAGTAG GACAAATCCG CCGGGAGCGG ATTTGAACGT 4151 TGCGAAGCAA CGGCCCGGAG GGTGGCGGGC AGGACGCCCG CCATAAACTG 4201 CCAGGCATCA AATTAAGCAG AAGGCCATCC TGACGGATGG CCTTTTTGCG 4251 TTTCTACAAA CTCTTTTGTT TATTTTTCTA AATACATTCA AATATGTATC 4301 CGCTCAT Sequence file prepared 02-November-2011.