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pFN19 and pFC20 HaloTag® T7 SP6 Flexi® Vectors

We are sorry, but these products are discontinued.

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This product is discontinued
pFN19 and pFC20 HaloTag® T7 SP6 Flexi® Vectors

The pFN19 and pFC20 HaloTag® T7 SP6 Flexi® Vectors feature inducible expression of HaloTag® fusion proteins in E. coli and T7 and SP6 cell-free systems. Expression levels depend highly on the nature of the protein, but in general the N-terminal HaloTag® fusion protein can increase expression level, enhance refolding and boost solubility of the expressed protein.

pFN19A/K HaloTag® T7 SP6 Flexi® Vectors (Cat.# G1891, G1841) are designed for protein expression with an N-terminal HaloTag® using the T7 or SP6 RNA polymerase promoter. pFC20A/K HaloTag® T7 SP6 Flexi® Vectors (Cat.# G1681, G1691) express protein with a C-terminal HaloTag® using the T7 and SP6 RNA polymerase promoter. Proteins expressed from these vectors can be purified using HaloTag® technology.

About the Flexi® Vector System

The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. Directly use recombinant clones and minimize time wasted screening background colonies. The versatile cloning of the Flexi® system means you can choose from a variety of expression systems and fusion tag orientations and then transfer to others as required. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector.

Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.

7498MA-W

pFN19A HaloTag® T7 SP6 Flexi® VectorGenBank® Accession Number EU545994 and vector sequence text file.

7499MA-W

pFN19K HaloTag® T7 SP6 Flexi® Vector GenBank® Accession Number EU545995 and vector sequence text file.

7500MA-W

pFC20A HaloTag® T7 SP6 Flexi® Vector GenBank® Accession Number EU545996 and vector sequence text file.

7501MA-W

pFC20K HaloTag® T7 SP6 Flexi® Vector GenBank® Accession Number EU545997 and vector sequence text file.

Specifications

You are viewing: G1891 Change Configuration

What's in the box?

Item Part # Size

pFN19A HaloTag® T7 SP6 Flexi® Vector

G189A 1 × 20μg

SDS

Choose language:

Download SDSPDF (196 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: G1841 Change Configuration

What's in the box?

Item Part # Size

pFN19K HaloTag® T7 SP6 Flexi® Vector

G184A 1 × 20μg

SDS

Choose language:

Download SDSPDF (196 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: G1681 Change Configuration

What's in the box?

Item Part # Size

pFC20A HaloTag® T7 SP6 Flexi® Vector

G168A 1 × 20μg

SDS

Choose language:

Download SDSPDF (195 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: G1691 Change Configuration

What's in the box?

Item Part # Size

pFC20K HaloTag® T7 SP6 Flexi® Vector

G169A 1 × 20μg

SDS

Choose language:

Download SDSPDF (195 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

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