The pALTER®-MAX Vector is a 5,534bp plasmid that contains the human cytomegalovirus (CMV) immediate-early enhancer/promoter region for strong, constitutive expression of cloned DNA inserts in many mammalian cell types. A chimeric intron composed of the 5´-splice site from the β-globin intron and the 3´-splice site from an IgG intron also enhances expression levels. Synthesize RNA in vitro using the T7 and T3 RNA polymerase promoters that flank the multiple cloning site to generate sense or antisense strands. The pALTER®-MAX Vector as supplied is chloramphenicol-resistant and ampicillin-sensitive.
Note: This product is a vector that was sold separately from the discontinued Altered Sites® II Mammalian Mutagenesis System (Cat.# Q5590). The pALTER®-MAX Vector is resistant to chloramphenicol but has an inactivated ampicillin resistance gene. These antibiotic resistance genes are for use in E. coli and not for stable expression in mammalian cells.
Please contact Technical Services if you have questions about using this vector.