We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Flexi® Enzyme Blend (SgfI & PmeI)

Reagent Used with Flexi® Cloning System

  • Required to screen Flexi® Vector clones for presence of protein-coding region of interest
  • Also can be used to cleave PCR products prior to cloning into Flexi® Vectors or transfer protein-coding regions between Flexi® Vectors

Size

Catalog number selected: R1851

$ 116.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
Flexi® Enzyme Blend (SgfI & PmeI)
25μl
$ 116.00
Your price: Log in
Change Configuration

The 10X Flexi® Enzyme Blend (SgfI and PmeI) is required to screen Flexi® Vector clones for the presence of the protein-coding region of interest. This enzyme blend can also be used to cleave PCR products prior to cloning into the Flexi® Vectors or transfer protein-coding regions between Flexi® Vectors. For more information see the Flexi® Vector Systems Technical Manual #TM254.

Recognition Sites:
SgfI recognition site:
5´…GCGATtCGC…3´
3´…CGCsTAGCG…5´

PmeI recognition site:
5´…GTTTtAAAC…3´
3´…CAAAsTTTG…5´

Storage Buffer: The 10X Flexi® Enzyme Blend (SgfI and PmeI) is supplied in 10mM Tris-HCl (pH 7.4 at 25°C), 150mM KCl, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.35mg/ml BSA, 0.05% Thesit® and 50% glycerol.

5X Flexi® Digest Buffer: The composition of 5X Flexi® Digest Buffer is 50mM Tris-HCl (pH 7.9 at 37°C), 50mM MgCl2, 250mM NaCl, 5mM DTT, 0.5mg/ml acetylated BSA.

Specifications

You are viewing: R1851 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Nuclease-Free Water

P119A 1 × 1,250μl View Product

Flexi® Enzyme Blend (SgfI & PmeI)

R185A 1 × 25μl

5X Flexi® Digest Buffer

R186A 1 × 1ml

SDS

Choose language:

Download SDSPDF (90 KB) – English (United States)

Certificate of Analysis

See all certificates for R1851

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 8,293,503, 9,018,014 and 8,367,403, European Pat. No. 1685247 and other patents and patents pending.

Specifications

You are viewing: R1852 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Nuclease-Free Water

P119A 2 × 1,250μl View Product

10X Flexi® Enzyme Blend (SgfI & PmeI)

R185B 1 × 100μl

5X Flexi® Digest Buffer

R186A 1 × 1ml

SDS

Choose language:

Download SDSPDF (90 KB) – English (United States)

Certificate of Analysis

See all certificates for R1852

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 8,293,503, 9,018,014 and 8,367,403, European Pat. No. 1685247 and other patents and patents pending.

Let's find the product that meets your needs.

Talk to a Scientist

Sylvia

Silvia

Italy