We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

TSAP Thermosensitive Alkaline Phosphatase

TSAP Thermosensitive Alkaline Phosphatase catalyzes the removal of 5´ phosphate groups from DNA, thus preventing the recircularization and religation of linearized cloning vector DNA during ligation. It is effective on 3´ overhangs, 5´ overhangs and blunt ends. It is also useful for preparing DNA for 5´ end-labeling by removing existing phosphate groups from the 5´ end.

TSAP is irreversibly inactivated by heating at 74°C for 15 minutes. Therefore, a DNA cleanup step is not required before proceeding to a ligation reaction. TSAP is fully active in all restriction enzyme reaction buffers tested under the conditio...

Expand to Read More »

  • *Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart

TSAP Thermosensitive Alkaline Phosphatase

100 units
- M9910 $ 69.00 Add to cart

QC Tests

Activity, SDS-PAGE/purity, endonuclease, DNase, RNase, blue/white cloning assay.



Storage Buffer

10mM Tris (pH 7.5 @ 25°C), 100mM NaCl, 50% glycerol.

Storage Conditions

Store at –20°C. See the expiration date on the label.

Unit Definition

One MBU is defined as the amount of enzyme required to dephosphorylate 1μg of pGEM® DNA with 3´ overhangs in 15 minutes at 37°C in a 50μl reaction using any Promega restriction enzyme buffer (except F). Dephosphorylation is defined as preventing >95% of the plasmid from ligating (analyzed by agarose gel electrophoresis).

For product intended use please see Patents & Disclaimers tab.

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.