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Promega Corporation

pSP64 Poly(A) Vector

The pSP64 Poly(A) Vector can be used as a standard cloning vector and for in vitro transcription from the SP6 promoter. The pSP64 Poly(A) Vector also can be used to generate poly(A)+ transcripts in vitro. The vector has a stretch of 30 dA:dT residues inserted between the SacI and EcoRI sites. Therefore, when foreign DNA is cloned into any polylinker site other than EcoRI (HindIII, PstI, SalI, AccI, HincII, XbaI, BamHI, AvaI, SmaI or SacI), linearization of the recombinant plasmid with EcoRI allows the use of SP6 RNA polymerase in vitro to prepare RNA copies of the inserted sequences that contain a synthetic 3´ "po...

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pSP64 Poly(A) Vector

- P1241 $ 104.00 Add to cart

GenBank®/EMBL Accession Number

pSP64 Poly(A) Vector Accession Number X65328; pSP64 Poly(A) Vector sequence text file.

Storage Conditions

Store vector at –20°C.

For product intended use please see Patents & Disclaimers tab.

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