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Promega Corporation

Alkaline Phosphatase, Calf Intestinal (CIAP)

Alkaline Phosphatase, Calf Intestinal (CIAP), catalyzes the hydrolysis of 5´-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vector DNA by removing phosphate groups from both 5´-termini and may also be used for the dephosphorylation of 5´ phosphorylated ends of DNA or RNA for subsequent labeling with [32P]ATP and T4 Polynucleotide Kinase. CIAP is active on 5´ overhangs, 5´ recessed and blunt ends.

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Alkaline Phosphatase, Calf Intestinal

1u/μl M1821 $ 81.00 Add to cart

Alkaline Phosphatase, Calf Intestinal (HC)

20u/μl M2825 $ 81.00 Add to cart

Available Separately

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CIAP Buffer Pack

(3 × 500μl)
- M1833 $ 24.00 Add to cart

QC Tests

Activity, endonuclease, DNase, RNase, blue/white cloning assay.


Calf intestinal mucosa.

Storage Buffer

10mM Tris-HCl (pH 8.0), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1μmol of 4-nitrophenyl phosphate per minute at 37°C in 1M diethanolamine, 10.9mM 4-nitrophenyl phosphate, 0.5mM MgCl2 (pH 9.8 at 25°C).

For product intended use please see Patents & Disclaimers tab.

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