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Bacterial Strains BMH 71-18 mutS and ES1301 mutS

Mismatch Repair Minus Strains of E. coli

  • Prevents repair of the newly synthesized unmethylated DNA strand
  • ES1301 is Tets for cycling between antibiotic resistances
  • BMH 71-18 is Tetr and can only be used to select Ampr or CATr mutants

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Catalog number selected: Q6131

$ 50.00
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Bacterial Strains BMH 71-18 mutS and ES1301 mutS
200μl
$ 50.00
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BMH 71-18 mutS and ES1301 mutS are mismatch repair minus E. colistrains. Use of these strains prevents repair of the newly synthesized unmethylated strand, leading to high mutation efficiencies. Both ES1301 and BMH 71-18 mutS are recA+, therefore inserts containing highly repetitive sequences may be unstable. ES1301 is Tets for cycling between antibiotic resistances. BMH 71-18 is Tetr; therefore, it can only be used to select Ampr or CATr mutants.

ES1301 mutS Genotype: lacZ53, mutS201::Tn5, thyA36, rha-5, metB1, deoC, IN(rrnD–rrnE).

BMH 71-18 mutS Genotype: thi, supE, Δ(lac-proAB), [mutS::Tn10], [F´, proAB, lacIqZΔM15].


Reference

  1. Wallace, R.B. et al. (1981) Gene 16, 21–6.

Specifications

You are viewing: Q6131 Change Configuration

What's in the box?

Item Part # Size

Bacterial Strain ES1301 mutS

Q613A 1 × 200μl

SDS

Choose language:

Download SDSPDF (42 KB) – English (United States)

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: Q6321 Change Configuration

What's in the box?

Item Part # Size

Bacterial Strain BMH 71-18 mutS

Q632A 1 × 500μl

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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Petra

Petra

Germany