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Bacterial Strains BMH 71-18 mutS and ES1301 mutS

BMH 71-18 mutS and ES1301 mutS are mismatch repair minus E. colistrains. Use of these strains prevents repair of the newly synthesized unmethylated strand, leading to high mutation efficiencies. Both ES1301 and BMH 71-18 mutS are recA+, therefore inserts containing highly repetitive sequences may be unstable. ES1301 is Tets for cycling between antibiotic resistances. BMH 71-18 is Tetr; therefore, it can only be used to select Ampr or CATr mutants.

ES1301 mutS Genotype: lacZ53, mutS201::Tn5, thyA36, rha-5, metB1, deoC, IN(rrnD–rrnE).

BMH 71-18 mutS Genotype: thi, supE, Δ(lac-proAB), [mutS::Tn10], [F´, proAB, lacIqZΔM15].


Reference

  1. Wallace, R.B. et al. (1981) Gene 16, 21–6.

Specifications

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What's in the box?

Item Part # Size

Bacterial Strain ES1301 mutS

Q613A 1 × 200μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: Q6321 Change Configuration

What's in the box?

Item Part # Size

Bacterial Strain BMH 71-18 mutS

Q632A 1 × 500μl

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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