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Bacterial Strains BMH 71-18 mutS and ES1301 mutS

Mismatch Repair Minus Strains of E. coli

  • Prevents repair of the newly synthesized unmethylated DNA strand
  • ES1301 is Tets for cycling between antibiotic resistances
  • BMH 71-18 is Tetr and can only be used to select Ampr or CATr mutants

Size

Catalog number selected: Q6131

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Bacterial Strains BMH 71-18 mutS and ES1301 mutS
200μl
$ 47.00
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BMH 71-18 mutS and ES1301 mutS are mismatch repair minus E. colistrains. Use of these strains prevents repair of the newly synthesized unmethylated strand, leading to high mutation efficiencies. Both ES1301 and BMH 71-18 mutS are recA+, therefore inserts containing highly repetitive sequences may be unstable. ES1301 is Tets for cycling between antibiotic resistances. BMH 71-18 is Tetr; therefore, it can only be used to select Ampr or CATr mutants.

ES1301 mutS Genotype: lacZ53, mutS201::Tn5, thyA36, rha-5, metB1, deoC, IN(rrnD–rrnE).

BMH 71-18 mutS Genotype: thi, supE, Δ(lac-proAB), [mutS::Tn10], [F´, proAB, lacIqZΔM15].


Reference

  1. Wallace, R.B. et al. (1981) Gene 16, 21–6.

Specifications

You are viewing: 200μl Change Configuration

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Item Part # Size

Bacterial Strain ES1301 mutS

Q613A 1 x 200μl

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: 500μl Change Configuration

What's in the box?

Item Part # Size

Bacterial Strain BMH 71-18 mutS

Q632A 1 x 500μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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