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Bacterial Strain LE392

Useful for Genomic and cDNA Cloning

  • Good primary strain for phage amplification or screening a cDNA library with nucleic acid probes
  • Permissive host that grows both recombinant and parental phage
  • Phage titers higher than Y1090 host cells

Size

Catalog number selected: K9981

$ 69.00 Your price: Log In

Bacterial Strain LE392
500μl
$ 69.00
Your price: Log In

Bacterial Strain LE392 is used for both genomic and cDNA cloning. LE392 lacks the E. coli K restriction system but is rec+. It is a permissive host, allowing both recombinant and parental phage to grow. Unlike Y1090, LE392 does not allow color selection of recombinants or IPTG induction of expression. Also, since LE392 contains lon protease activity, fusion proteins are often less stable in this host than in Y1090. When using this strain, phage titers tend to be higher than with Y1090 host cells. Therefore, we recommend (if color selection is not required) that LE392 be the primary strain for amplification of recombinant phage and for screening a cDNA library with nucleic acid probes.

Genotype: hsdR514(rk, mk+), glnV(supE44), tryT (supF58), lacY1 or Δ(lacIZY)6, galK2, galT22, metB1, trpR55.

Specifications

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Item Part # Size

Bacterial Strain LE392

K998A 1 × 0.5ml

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA
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