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NanoLuc®-DMPK Fusion Vector

NV3021_NanoLuc--DMPK Fusion Vector- 20ug
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NanoLuc®-DMPK Fusion Vector

Express Full-Length NanoLuc®-Kinase Fusions in Mammalian Cells

The NanoLuc®-DMPK Fusion Vector contains the coding region of NanoLuc® luciferase fused to the N-terminus of human DMPK kinase. It is designed for use with the NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay, Adherent Format protocol, where the plasmid can be transfected into various cell lines for DMPK target engagement analysis. The NanoLuc® luciferase kinase fusion vectors are supplied transfection-ready. The CMV promoter drives the expression of the full-length NanoLuc® kinase fusion protein. 

Vector Details:

  • Gene name: DMPK
  • Sequence
  • Recommended Assay: NanoBRET™ TE Intracellular Kinase, K-10
  • Example data using Tracer: K-10

About Kinase Fusion Vector Names

The placement of NanoLuc® either 5´ or 3´ of the kinase gene is optimized for use with NanoBRET™ TE Assays and reflected in the vector name. For example, NanoLuc®-kinase implies that NanoLuc® is 5´ to the kinase gene (at the N-terminus of the kinase). Similarly, kinase-NanoLuc® implies NanoLuc® is 3´to the kinase gene (at the C-terminus of the kinase).

NanoBRET™ Target Engagement (TE) Intracellular Kinase Assays

The NanoBRET™ TE Intracellular Kinase Assays can analyze the affinity of test compounds by competitive displacement of a fluorescent NanoBRET™ Tracer reversibly bound to a NanoLuc®-kinase fusion protein in cells. Introduction of competing test compounds results in a dose-dependent decrease in the NanoBRET™ signal for estimating intracellular affinity against the target protein. The NanoBRET™ Tracer used is determined by your choice of NanoLuc® kinase fusion vector. The Kinase Vector Selection table shows which NanoBRET™ Tracer assay is correct for each kinase fusion vector.


What's in the box?

Item Part # Size Concentration

NanoLuc®-DMPK Fusion Vector

NV302A 1 × 20μg 1μg/μl

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


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