We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

SAM2® Biotin Capture Membrane

Streptavidin Matrix for Capture of Biotinylated Substrates

  • Allows kinetic studies
  • Rapid, strong substrate binding
  • Minimal non-specific binding

Introducing the fastest way to order everything you need.

Learn more ›

Size

Catalog number selected: V2861

$ 355.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
SAM2® Biotin Capture Membrane
96 samples
$ 355.00
Your price: Log in

Quantitative Binding of Biotinylated Substrates 

The SAM Biotin Capture Membrane binds biotinylated molecules based on their affinity for streptavidin. The proprietary process by which the SAM Membrane is produced results in a high density of streptavidin on the filter, providing rapid, quantitative substrate binding in the nmol/cm2 range, depending upon the substrate used. Applications include quantitation of protein kinase activity, cytokines, specific genes or mRNA. 

Available as a large, prenumbered, partially cut sheet (approximately 10.5 × 15.0cm), the membrane can be easily separated into 96 individual squares and is designed for small-scale experiments where high binding capacity is required.

The membrane may be analyzed using phosphorimaging analysis, autoradiography or scintillation counting to quantitate results. The membrane also has been used successfully with chemiluminescence detection techniques. The use of fluorescence for detection of captured molecules is not recommended.

Advantages

  • Use a variety of substrates: Different substrate types can be analyzed without the need to optimize each substrate for binding to a matrix. You can perform experiments with a wide array of sample numbers and sizes without changing the analysis technique.
  • Minimal nonspecific binding: The combination of protein denaturant and high-salt washes minimizes nonspecific binding to the membrane without interfering with the high-affinity interaction between streptavidin and biotin.
  • High signal-to-noise ratios: The stringent washing conditions result in low background counts.
  • Allows kinetic studies: Membrane can linearly bind biotinylated substrates up to the nmol/cmrange.
  • Strong binding reaction: Membrane retains the biotin conjugate over 8 logs of pH (pH 2–10), changes in temperature, organic solvents, ionic and nonionic detergents (SDS, CHAPS, Triton® X-100, Tween® 20 and Tween® 80) and denaturing agents (5M guanidine-HCl and 2M urea).
  • Rapid binding: Binds within 1 minute.

Specifications

SDS

Choose language:

Download SDSPDF (40 KB) – English (United States)

Certificate of Analysis

See all certificates for V2861

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Store membranes at –20°C in resealable bag.

Patents and Disclaimers

U.S. Pat. Nos. 6,066,462, 6,348,310 and 6,753,157.

Let's find the product that meets your needs.

Talk to a Scientist

Petra

Petra

Germany