The SAM2® Biotin Capture Membrane binds biotinylated molecules based on their affinity for streptavidin. The proprietary process by which the SAM2® Membrane is produced results in a high density of streptavidin on the filter, providing rapid, quantitative substrate binding in the nmol/cm2 range, depending upon the substrate used. Applications include quantitation of protein kinase activity, cytokines, specific genes or mRNA.
Available as a large, prenumbered, partially cut sheet (approximately 10.5 × 15.0cm), the membrane can be easily separated into 96 individual squares and is designed for small-scale experiments where high binding capacity is required.
The membrane may be analyzed using phosphorimaging analysis, autoradiography or scintillation counting to quantitate results. The membrane also has been used successfully with chemiluminescence detection techniques. The use of fluorescence for detection of captured molecules is not recommended.