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SAM2® Biotin Capture Membrane

Streptavidin Matrix for Capture of Biotinylated Substrates

  • Allows kinetic studies
  • Rapid, strong substrate binding
  • Minimal non-specific binding

Size

Catalog number selected: V2861

$ 325.00 Your price: Log In

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SAM2® Biotin Capture Membrane
96 samples
$ 325.00
Your price: Log In

Quantitative Binding of Biotinylated Substrates 

The SAM Biotin Capture Membrane binds biotinylated molecules based on their affinity for streptavidin. The proprietary process by which the SAM Membrane is produced results in a high density of streptavidin on the filter, providing rapid, quantitative substrate binding in the nmol/cm2 range, depending upon the substrate used. Applications include quantitation of protein kinase activity, cytokines, specific genes or mRNA. 

Available as a large, prenumbered, partially cut sheet (approximately 10.5 × 15.0cm), the membrane can be easily separated into 96 individual squares and is designed for small-scale experiments where high binding capacity is required.

The membrane may be analyzed using phosphorimaging analysis, autoradiography or scintillation counting to quantitate results. The membrane also has been used successfully with chemiluminescence detection techniques. The use of fluorescence for detection of captured molecules is not recommended.

Advantages

  • Use a variety of substrates: Different substrate types can be analyzed without the need to optimize each substrate for binding to a matrix. You can perform experiments with a wide array of sample numbers and sizes without changing the analysis technique.
  • Minimal nonspecific binding: The combination of protein denaturant and high-salt washes minimizes nonspecific binding to the membrane without interfering with the high-affinity interaction between streptavidin and biotin.
  • High signal-to-noise ratios: The stringent washing conditions result in low background counts.
  • Allows kinetic studies: Membrane can linearly bind biotinylated substrates up to the nmol/cmrange.
  • Strong binding reaction: Membrane retains the biotin conjugate over 8 logs of pH (pH 2–10), changes in temperature, organic solvents, ionic and nonionic detergents (SDS, CHAPS, Triton® X-100, Tween® 20 and Tween® 80) and denaturing agents (5M guanidine-HCl and 2M urea).
  • Rapid binding: Binds within 1 minute.

Protocols

Specifications

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Store membranes at –20°C in resealable bag.

Patents and Disclaimers

U.S. Pat. Nos. 6,066,462, 6,348,310 and 6,753,157.

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