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Protein Kinase C

Purified PKC for use in Kinase Assays

  • Greater than 90% pure as determined by SDS-PAGE
  • Primarily α, β and γ isoforms with lesser amounts of δ and ζ isoforms
  • Supplied at a concentration of 25μg/ml

Size

Catalog number selected: V5261

$ 350.00 Your price: Log In

Protein Kinase C
1μg
$ 350.00
Your price: Log In

Protein Kinase C is an 82kDa monomeric enzyme consisting of a C-terminal catalytic domain and a cysteine-rich N-terminal regulatory domain. The regulatory domain contains the sites for calcium and phospholipid binding and a pseudosubstrate subdomain, the target for PKC autophosphorylation. PKC is isolated from rat brain following the procedure of Walton and colleagues. The purified PKC consists primarily of α, β and γ isoforms with lesser amounts of δ and ζ isoforms.

Storage Buffer: 20mM Tris-HCl (pH 7.5), 2mM EGTA, 2mM EDTA, 1mM DTT, 10mM potassium phosphate (pH 7.5), 0.05% Triton® X-100 and 50% glycerol.

Unit Definition: One unit is the amount of kinase needed to transfer 1nmol of phosphate per minute using Type III-S histone protein as the substrate at 30°C in the presence of 0.6mg/ml phosphatidyl serine.

Reference

Walton, G.M. et al. (1987) Anal. Biochem. 161, 425–37.

Protocols

Specifications

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Item Part # Size

Protein Kinase C

V526A 2 x 0.5μg

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Store at –70°C.

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