The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The PKC Kinase Enzyme System contains:
- PKC Kinase, 0.5μg (rat brain, native full-length). MW: various (contains α, β and γ isoforms with lesser amounts of δ and ζ isoforms).
- Neurogranin(28–43) (PKC) Peptide Substrate (AAKIQASFRGHMARKK).
- PKC Activation 5X Buffer, PKC Coactivation Buffer.
Native rat brain Protein Kinase C (PKC) is purified according to the method of Walton et al. The purified PKC consists primarily of α, β and γ isoforms with lesser amounts of δ and ζ isoforms. The gene accession number for PKCα is NM_001105713.1.
PKC NCBI Database Entry.
The PKC Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
See all Kinase Enzyme Systems available from Promega.