PI3K Lipid Kinases

Purified PI3K Class I Lipid Kinases

  • Use with ADP-Glo™ Assay and PI3K Substrates in lipid kinase activity assays

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Catalog number selected: V1721

$ 475.00
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PI3K Lipid Kinases
p110α/p85α/200µl
$ 475.00
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Recombinant Human Phosphoinositide 3-Kinases (PI3Ks)

Phosphatidylinositol (PI) and its phosphorylated derivates, collectively called phosphoinositides, are important second messengers that are critical as signaling molecules and for cellular membrane remodeling. These derivatives are generated by a family of kinases called phosphoinositide lipid kinases (PIKs). 

We offer lipid kinase enzymes, substrates and detection systems for performing phosphoinositide lipid kinase (PIK) reactions using a luminescent ADP-detection platform, the ADP-Glo™ Kinase Assay. The purified human recombinant PI3K enzymes are components of the PI3K Class I Profiling Kit (Cat.# V1690) that are available separately. 

Sources

PI3K (p110α/p85α), 20μg: Complex of recombinant N-terminal FLAG-tagged full-length human p110α (GenBank® Accession Number U79143) and human p85α (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.

PI3K (p110α[E545K]/p85α), 20μg:
Complex of recombinant N-terminal FLAG-tagged full-length human p110α (GenBank® Accession Number U79143) and human p85α (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.

PI3K (p110α[H1047R]/p85α), 20μg:
Complex of recombinant N-terminal FLAG-tagged full-length human p110α (GenBank® Accession Number U79143) and human p85α (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.

PI3K (p110β/p85α), 20μg: Complex of recombinant N-terminal FLAG-tagged full-length human p110β (GenBank® Accession Number NM_006219) and human p85α (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.

PI3K (p120γ), 20μg:
Recombinant N-terminal His-tagged full-length human p120γ (GenBank® Accession Number NM _02649) expressed in Sf9 insect cells.

PI3K (p110δ/p85α), 20μg:
Complex of recombinant N-terminal GST-tagged full-length human p110δ (GenBank® Accession Number NM_005026) and human p85α (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.

Specifications

You are viewing: V1721 Change Configuration

What's in the box?

Item Part # Size
PI3K Reaction Buffer, 5X V169A 1 × 1ml
PI3K (p110α/p85α), 20µg V172B 1 × 200μl

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Technical Manual for detailed storage recommendations.

Specifications

You are viewing: V1731 Change Configuration

What's in the box?

Item Part # Size
PI3K Reaction Buffer, 5X V169A 1 × 1ml
PI3K (p110α[E545K]/p85α), 20µg V173B 1 × 200μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Technical Manual for detailed storage recommendations.

Specifications

You are viewing: V1741 Change Configuration

What's in the box?

Item Part # Size
PI3K Reaction Buffer, 5X V169A 1 × 1ml
PI3K (p110α[H1047R]/p85α), 20µg V174B 1 × 200μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Technical Manual for detailed storage recommendations.

Specifications

You are viewing: V1751 Change Configuration

What's in the box?

Item Part # Size
PI3K Reaction Buffer, 5X V169A 1 × 1ml
PI3K (p110β/p85α), 20µg V175B 1 × 200μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Technical Manual for detailed storage recommendations.

Specifications

You are viewing: V1761 Change Configuration

What's in the box?

Item Part # Size
PI3K Reaction Buffer, 5X V169A 1 × 1ml
PI3K (p120γ), 20µg V176B 1 × 200μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Technical Manual for detailed storage recommendations.

Specifications

You are viewing: V1771 Change Configuration

What's in the box?

Item Part # Size
PI3K Reaction Buffer, 5X V169A 1 × 1ml
PI3K (p110δ/p85α), 20µg V177B 1 × 200μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Technical Manual for detailed storage recommendations.

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