InCELLect™ St-Ht31P Control Peptide is a stearated form of the peptide Ht-31P. This peptide is used as a negative control peptide with the InCELLect™ AKAP St-Ht31 Inhibitor Peptide, which inhibits the interaction between protein kinase A and AKAP. The Control Peptide has a similar sequence to the Inhibitor Peptide but does not inhibit the interaction.
The presence of the hydrophobic stearated moiety enhances the cellular uptake of the peptide, allowing it to be used to study PKA signaling in intact cells in culture.
- Concentration: 10mM.
- Purity: >80% by HPLC.
- Molecular Weight: 2,765±3 a.m.u.
Optimal concentration and incubation times will need to be determined for each experimental system. Reference 1 describes the inhibitory effects for the InCELLect™ AKAP St-Ht31 Inhibitor Peptide in the range of 5–50μM within 3–5 minutes after treatment.
- Vijayaraghavan, S. et al. (1997) J. Biol. Chem. 272, 4747–52.