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cGMP-Dependent Protein Kinase

Purified Kinase for Protein Phosphorylation, Regulation and Signal Transduction Studies

  • >90% pure as determined by SDS-PAGE (single band)
  • Use in studies of cGMP-dependent protein kinase substrates and inhibitors

Size

Catalog number selected: V5171

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cGMP-Dependent Protein Kinase
6,000u
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cGMP-Dependent Protein Kinase is a serine/threonine protein kinase present in smooth muscle and a variety of other tissues. The kinase is a 78kDa polypeptide composed of a regulatory domain and a catalytic domain and is active as a homodimer.

Use cGMP-Dependent Protein Kinase to investigate the role of target protein phosphorylation in cell regulation and signal transduction. Applications include microinjection studies of the role of cGMP-Dependent Protein Kinase in cellular processes;  in vitro enzymological studies of protein phosphorylation; and studies on cGMP-Dependent Protein Kinase substrates and inhibitors. 

Storage Buffer: 10mM potassium phosphate (pH 6.8), 1mM EDTA, 25mM β-mercaptoethanol, 0.15M NaCl, 12% sucrose.

Unit Definition: One unit is defined as the amount of cGMP-Dependent Protein Kinase required to catalyze incorporation of 1pmol of phosphate into a heptapeptide substrate (RKRSRAE) in one minute at 30°C.

Properties:

  • Specific Activity: >1,000u/μg (kinase activity).
  • Highly Pure: cGMP-Dependent Protein Kinase has been purified by the method of Corbin and Doskeland and is >90% pure as determined by SDS-PAGE (single band).

Reference:

  1. Corbin, J.D. and Doskeland, S.O. (1983) J. Biol. Chem. 258, 11391–7.

Specifications

What's in the box?

Item Part # Size

cGMP-Dependent Protein Kinase (α Isozyme)

V517A 1 × 6,000u

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Store at –70°C.

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