cGMP-Dependent Protein Kinase is a serine/threonine protein kinase present in smooth muscle and a variety of other tissues. The kinase is a 78kDa polypeptide composed of a regulatory domain and a catalytic domain and is active as a homodimer.
Use cGMP-Dependent Protein Kinase to investigate the role of target protein phosphorylation in cell regulation and signal transduction. Applications include microinjection studies of the role of cGMP-Dependent Protein Kinase in cellular processes; in vitro enzymological studies of protein phosphorylation; and studies on cGMP-Dependent Protein Kinase substrates and inhibitors.
Storage Buffer: 10mM potassium phosphate (pH 6.8), 1mM EDTA, 25mM β-mercaptoethanol, 0.15M NaCl, 12% sucrose.
Unit Definition: One unit is defined as the amount of cGMP-Dependent Protein Kinase required to catalyze incorporation of 1pmol of phosphate into a heptapeptide substrate (RKRSRAE) in one minute at 30°C.
- Specific Activity: >1,000u/μg (kinase activity).
- Highly Pure: cGMP-Dependent Protein Kinase has been purified by the method of Corbin and Doskeland and is >90% pure as determined by SDS-PAGE (single band).
- Corbin, J.D. and Doskeland, S.O. (1983) J. Biol. Chem. 258, 11391–7.