Anti-ACTIVE® MAPK pAb is a polyclonal rabbit antibody that preferentially detects the dually phosphorylated, active form of the mitogen-activated protein kinase (MAPK) enzymes (ERK1 and ERK2). The antibody is affinity purified using a dually phosphorylated peptide that corresponds to the active form of the mitogen-activated protein (MAP) kinase enzymes.
When used at the recommended 1:5,000 dilution, this product will generate 200ml of blotting solution, sufficient for 20 Western blots of 10ml each.
- Immunogen: Dually phosphorylated Thr/Glu/Tyr region (pTEpY) derived from the catalytic core of the active form of the mitogen-activated protein kinase (MAPK) enzymes, ERK1 and ERK2, which corresponds to Thr183 and Tyr185 of the mammalian ERK2 enzyme.
- Antibody Form: Affinity-purified rabbit IgG; supplied in PBS (pH 7.4).
- Working Dilutions: Western blotting (1:5,000); Immunocytochemistry (1:500); Immunohistochemistry (1:500).