Anti-ACTIVE® JNK pAb is a polyclonal antibody that preferentially detects the dually phosphorylated, active form of the stress-activated protein kinase (SAPK), also known as c-Jun N-terminal kinase, JNK.
The antibody is affinity purified using a dually phosphorylated peptide that corresponds to the active form of the JNK enzymes.
Anti-ACTIVE® JNK pAb is available in two convenient sizes. Cat.# V7931 will generate up to 200ml of blotting solution, sufficient for 20 Western blots of 10ml each. The larger size, Cat.# V7932, will generate up to 600ml of blotting solution, sufficient for 60 Western blots of 10ml each.
- Immunogen: Dually phosphorylated Thr/Pro/Tyr region (pTPpY) derived from the catalytic core of the active form of JNK kinase, which corresponds to Thr183 and Tyr185 of the mammalian JNK2 enzyme.
- Antibody Form: Affinity-purified rabbit IgG; supplied in 10mM sodium phosphate (pH 7.4), 20mM NaCl.
- Working Dilutions: Western blotting (1:5,000); Immunocytochemistry (1:1,000); Immunohistochemistry (1:500).