The ADP-Glo™ Kinase Assay with PI:3PS (Cat.# V1781, V1782) contains PI:3PS Lipid Kinase Substrate (0.25mg) and the ADP-Glo™ Kinase Assay.
The ADP-Glo™ Kinase Assay with PIP2:3PS (Cat.# V1791, V1792) contains PIP2:3PS Substrate (0.25mg) and the ADP-Glo™ Kinase Assay.
These products are designed for use in screening assays with purified PI3K Class I lipid kinases. The lipid substrates are supplied as frozen small unilamellar vesicles containing a mixture of phosphatidylinositol (PI) or phosphoinositol-4,5-bisphosphate (PIP2) at a 1:3 ratio with phosphatidylserine (PS) as carrier lipid. The PIP2:3PS substrate is optimized for use with class I PI3Ks. The PI:3PS substrate provides a universal PI lipid kinase substrate.
The ADP-Glo™ Kinase Assay with PI:3PS and the ADP-Glo™ Kinase Assay with PIP2:3PS are components of the PI3K-Glo™ Class I Profiling Kit (Cat.# V1690).