We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Kinase Selectivity Profiling Systems—AGC

Selectivity Screening of Library Compounds for Effect on AGC Family Kinases

  • Kinase and substrate pairs organized in an easy-to-use 8-tube strip format
  • Simple reaction assembly and automation–friendly protocol
  • Streamilines profiling of hits and leads for potency against families of related kinases

Choose a kinase profiling system

Additional options

Catalog number selected: V6858

$ 554.00
Your price:
Add to Cart
This product is discontinued
Kinase Selectivity Profiling Systems—AGC
AGC-1
$ 554.00
Your price: Log in
Change Configuration
32460619-overview

Profile Lead Compounds in Hours, Not Days

The Kinase Selectivity Profiling Systems are ideal for profiling lead-compound kinase inhibitors against a broad panel of kinases to better understand inhibitor activity and obviate any off-target effects. Each system includes kinase and substrate pairs organized in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.

The AGC1 and AGC2 Kinase Selectivity Profiling Systems contain sets of kinases from the AGC subfamily of protein kinases. Each profiling system contains the reagents needed to complete the profile, including kinase reaction buffer, eight kinases in each multiwell strip and eight corresponding substrates and cofactors in another multiwell strip.

Optimized for Use With ADP-Glo™ Kinase Assay

The Kinase Selectivity Profiling Systems rely on the luminescent ADP-Glo™ Kinase Assay technology to characterize kinase selectivity. The luminescence generated by the ADP-Glo™ Kinase Assay is proportional to ADP concentration and correlates with kinase activity.

Each Kinase Selectivity Profiling System is available with or without ADP-Glo™ Assay reagents.

32460619-starred-callout

Simple, Automation-Friendly Assay, No Time-Consuming Optimization

Simply dilute the stock solutions provided, and you’re ready to go.  Two quick, one-step dilutions provide working stocks of kinases and substrate/cofactor solutions. 

Concentrated kinases and substrates/cofactor stocks are first diluted and then combined with test compound in a 384-well plate. After a 1-hour incubation, the ADP-Glo™ Assay is performed to measure kinase activity.

Kinase profiling method overview 12059ma-w
32460619-assay-performance

Assays can be performed manually, or automated on a liquid-handler.

Single-Dose Profiling

Each Kinase Selectivity Profiling System provides enough material to profile up to 20 compounds at a single dose or create a dose response for two compounds against eight kinases at once. 
12060MB-W
Example single-dose inhibitor profiles of eight compounds created using the Kinase Selectivity Profiling System—TK-3. Kinase assays were assembled in the presence of the indicated compounds (1μM final concentration). Kinase activity was quantified using the ADP-Glo™ Kinase Assay. The percent activity of each kinase in the presence of each compound is shown.
32460619-custom-kinase

Custom Kinase Assay Strips Available

If you need a different combination of kinases than those provided in our catalog, you can design your own custom profiling system using our kinase selector tool.

Specifications

You are viewing: V6858 Change Configuration

SDS

Choose language:

Download SDSPDF (287 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Specifications

You are viewing: V6859 Change Configuration

What's in the box?

Item Part # Size

Kinase Selectivity Profiling System: AGC-1

V6858 1 × 8 × 50 reactions

ADP-Glo™ Kinase Assay

V6930 1 × 400 assays

SDS

Choose language:

Download SDSPDF (385 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

Specifications

You are viewing: V6910 Change Configuration

SDS

Choose language:

Download SDSPDF (264 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Specifications

You are viewing: V6931 Change Configuration

What's in the box?

Item Part # Size

Kinase Selectivity Profiling System: AGC-2

V6910 1 × 8 × 50 reactions

ADP-Glo™ Kinase Assay

V6930 1 × 400 assays

SDS

Choose language:

Download SDSPDF (369 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

Let's find the product that meets your needs.

Talk to a Scientist

Manas

Manas

India