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Promega Corporation

SIRT-Glo™ Assays and Screening Systems

The SIRT-Glo™ Assays and Screening Systems are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of the NAD+-dependent histone deacetylase (HDAC) class III enzymes (sirtuins; SIRTs) from purified enzyme sources. The assays use an acetylated, luminogenic peptide substrate that can be deacetylated by SIRT activities. Deacetylation of the peptide aminoluciferin substrate is measured using a coupled enzymatic system in which a protease in the Developer Reagent cleaves the deacetylated peptide from the aminoluciferin substrate, releasing aminoluciferin, which is quantified in...

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  • *Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart

SIRT-Glo™ Assay

10ml
- G6450 $ 460.00 Add to cart

SIRT-Glo™ Screening System

5 × 10ml
- G6471 Please Enquire


Available Separately

Product Size Conc. Catalog # *List Price QTY Add to Cart

Nicotinamide

30μl
1M G6540 $ 95.00 Add to cart

HeLa Nuclear Extract

10μl
5mg/ml G6570 $ 135.00 Add to cart

Storage Conditions

Store the SIRT-Glo™ Assay components and SIRT-Glo™ Control Substrate at –20°C. Store HeLa Nuclear Extract at –70°C.

For product intended use please see Patents & Disclaimers tab.

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