pGloSensor(TM)-22F cAMP Plasmid This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGloSensor(TM)-22F cAMP Vector sequence reference points: Base pairs 6990 Synthetic beta-lactamase (Ampr) coding region 5-865 Synthetic poly(A) signal/transcriptional pause region 970-1123 Reporter Vector primer 3 (RVprimer3) binding region 1072-1091 CMV immediate early enhancer/promoter 1137-1878 T7 RNA polymerase promoter (-17 to +3) 1890-1909 pGloSensor(TM)-22F cAMP biosensor-coding region 1922-4027 SV40 late polyadenylation signal 4060-4281 SV40 early enhancer/promoter 4329-4747 Synthetic poly(A) signal/transcriptional pause region 5833-5881 Reporter Vector primer 4 (RVprimer4) binding region 5948-5967 ColE1-derived plasmid origin of replication 6205-6241 .. 1 GTGGTTACCA GTGCTTGATC AGTGAGGCAC CGATCTCAGC GATCTGCCTA 51 TTTCGTTCGT CCATAGTGGC CTGACTCCCC GTCGTGTAGA TCACTACGAT 101 TCGTGAGGGC TTACCATCAG GCCCCAGCGC AGCAATGATG CCGCGAGAGC 151 CGCGTTCACC GGCCCCCGAT TTGTCAGCAA TGAACCAGCC AGCAGGGAGG 201 GCCGAGCGAA GAAGTGGTCC TGCTACTTTG TCCGCCTCCA TCCAGTCTAT 251 GAGCTGCTGT CGTGATGCTA GAGTAAGAAG TTCGCCAGTG AGTAGTTTCC 301 GAAGAGTTGT GGCCATTGCT ACTGGCATCG TGGTATCACG CTCGTCGTTC 351 GGTATGGCTT CGTTCAACTC TGGTTCCCAG CGGTCAAGCC GGGTCACATG 401 ATCACCCATA TTATGAAGAA ATGCAGTCAG CTCCTTAGGG CCTCCGATCG 451 TTGTCAGAAG TAAGTTGGCC GCGGTGTTGT CGCTCATGGT AATGGCAGCA 501 CTACACAATT CTCTTACCGT CATGCCATCC GTAAGATGCT TTTCCGTGAC 551 CGGCGAGTAC TCAACCAAGT CGTTTTGTGA GTAGTGTATA CGGCGACCAA 601 GCTGCTCTTG CCCGGCGTCT ATACGGGACA ACACCGCGCC ACATAGCAGT 651 ACTTTGAAAG TGCTCATCAT CGGGAATCGT TCTTCGGGGC GGAAAGACTC 701 AAGGATCTTG CCGCTATTGA GATCCAGTTC GATATAGCCC ACTCTTGCAC 751 CCAGTTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC GGGGTGTGCA 801 AAAACAGGCA AGCAAAATGC CGCAAAGAAG GGAATGAGTG CGACACGAAA 851 ATGTTGGATG CTCATACTCG TCCTTTTTCA ATATTATTGA AGCATTTATC 901 AGGGTTACTA GTACGTCTCT CAAGGATAAG TAAGTAATAT TAAGGTACGG 951 GAGGTATTGG ACAGGCCGCA ATAAAATATC TTTATTTTCA TTACATCTGT 1001 GTGTTGGTTT TTTGTGTGAA TCGATAGTAC TAACATACGC TCTCCATCAA 1051 AACAAAACGA AACAAAACAA ACTAGCAAAA TAGGCTGTCC CCAGTGCAAG 1101 TGCAGGTGCC AGAACATTTC TCTGGCCTAA CTGGCCTCAA TATTGGCCAT 1151 TAGCCATATT ATTCATTGGT TATATAGCAT AAATCAATAT TGGCTATTGG 1201 CCATTGCATA CGTTGTATCT ATATCATAAT ATGTACATTT ATATTGGCTC 1251 ATGTCCAATA TGACCGCCAT GTTGGCATTG ATTATTGACT AGTTATTAAT 1301 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC 1351 GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC 1401 CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 1451 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC 1501 TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTCCGCCCC CTATTGACGT 1551 CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAC 1601 GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC 1651 ATGGTGATGC GGTTTTGGCA GTACACCAAT GGGCGTGGAT AGCGGTTTGA 1701 CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT 1751 TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA TAACCCCGCC 1801 CCGTTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 1851 CAGAGCTCGT TTAGTGAACC GTCAGATCAC TAGAAGCTTT AATACGACTC 1901 ACTATAGGGC TAGCGATCGC CATGCCTGGC GCAGTAGGCA AGGTGGTGCC 1951 CTTCATCGAG GCTAAGGTGG TGGACTTGGA CACTGGTAAG ACACTGGGTG 2001 TGAACCAGCG CGGCGAGCTG TGCGTCCGTG GCCCCATGAT CATGAGCGGC 2051 TACGTTAACA ACCCCGAGGC TACAAACGCT CTCATCGACA AGGACGGCTG 2101 GCTGCACAGC GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA 2151 TCGTGGACCG GCTGAAGAGC CTGATCAAAT ACAAGGGCTA CCAGGTAGCC 2201 CCAGCCGAAC TGGAGAGCAT CCTGCTGCAA CACCCCAACA TCTTCGACGC 2251 CGGGGTCGCC GGCCTGCCCG ACGACGATGC CGGCGAGCTG CCCGCCGCAG 2301 TCGTCGTGCT GGAACACGGT AAAACCATGA CCGAGAAGGA GATCGTGGAC 2351 TATGTGGCCA GCCAGGTTAC AACCGCCAAG AAGCTGCGCG GTGGTGTTGT 2401 GTTAGTGGAC GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA 2451 AGATCCGCGA GATTCTCATT AAGGCCAAGA AGGGATCCAA TTGGGATTCT 2501 GGGTGCTCCA GAGAAGGTAT GTATGAAAGC TTTATTGAGT CACTGCCATT 2551 CCTTAAATCT TTGGAGTTTT CTGCACGCCT GAAAGTAGTA GATGTGATAG 2601 GCACCAAAGT ATACAACGAT GGAGAACAAA TCATTGCTCA GGGAGATTCG 2651 GCTGATTCTT TTTTCATTAT TGAATCTGGA GAAGTGAAAA TTACTATGAA 2701 AAGAAAGGGT AAATCAGAAG TGGAAGAGAA TGGTGCAGTA GAAATCGCTC 2751 GATGCTCGCG GGGACAGTAC TTTGGAGAGC TTGCCCTGGT AACTAACAAA 2801 CCTCGAGCAG CTTCTGCCCA CGCCATTGGG ACTGTCAAAT GTTTAGCAAT 2851 GGATGTGCAA GCATTTGAAA GGCTTCTGGG ACCTTGCATG GAAATTATGA 2901 AAAGGAACAT CGCTACCTAT GAAGAACAGT TAGTTGCCCT GTATGGAACG 2951 AACATGGATA TTGTAGCCAA AAACATTAAG AAGGGCCCAG CGCCATTCTA 3001 CCCACTCGAA GACGGGACCG CCGGCGAGCA GCTGCACAAA GCCATGAAGC 3051 GCTACGCCCT GGTGCCCGGC ACCATCGCCT TTACCGACGC ACATATCGAG 3101 GTGGACATTA CCTACGCCGA GTACTTCGAG ATGAGCGTTC GGCTGGCAGA 3151 AGCTATGAAG CGCTATGGGC TGAATACAAA CCATCGGATC GTGGTGTGCA 3201 GCGAGAATAG CTTGCAGTTC TTCATGCCCG TGTTGGGTGC CCTGTTCATC 3251 GGTGTGGCTG TGGCCCCAGC TAACGACATC TACAACGAGC GCGAGCTGCT 3301 GAACAGCATG GGCATCAGCC AGCCCACCGT CGTATTCGTG AGCAAGAAAG 3351 GGCTGCAAAA GATCCTCAAC GTGCAAAAGA AGCTACCGAT CATACAAAAG 3401 ATCATCATCA TGGATAGCAA GACCGACTAC CAGGGCTTCC AAAGCATGTA 3451 CACCTTCGTG ACTTCCCATT TGCCACCCGG CTTCAACGAG TACGACTTCG 3501 TGCCCGAGAG CTTCGACCGG GACAAAACCA TCGCCCTGAT CATGAACAGT 3551 AGTGGCAGTA CCGGATTGCC CAAGGGCGTA GCCCTACCGC ACCGCACCGC 3601 TTGTGTCCGA TTCAGTCATG CCCGCGACCC CATCTTCGGC AACCAGATCA 3651 TCCCCGACAC CGCTATCCTC AGCGTGGTGC CATTTCACCA CGGCTTCGGC 3701 ATGTTCACCA CGCTGGGCTA CTTGATCTGC GGCTTTCGGG TCGTGCTCAT 3751 GTACCGCTTC GAGGAGGAGC TATTCTTGCG CAGCTTGCAA GACTATAAGA 3801 TTCAATCTGC CCTGCTGGTG CCCACACTAT TTAGCTTCTT CGCTAAGAGC 3851 ACTCTCATCG ACAAGTACGA CCTAAGCAAC TTGCACGAGA TCGCCAGCGG 3901 CGGGGCGCCG CTCAGCAAGG AGGTAGGTGA GGCCGTGGCC AAACGCTTCC 3951 ACCTACCAGG CATCCGCCAG GGCTACGGCC TGACAGAAAC AACCAGCGCC 4001 ATTCTGATCA CTCCAGAAGG GGTTTAAACT CTAGAGTCGG GGCGGCCGGC 4051 CGCTTCGAGC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA 4101 ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT 4151 TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA 4201 ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT 4251 TAAAGCAAGT AAAACCTCTA CAAATGTGGT AAAATCGATA AGGATCCGTT 4301 TGCGTATTGG GCGCTCTTCC GCTGATCTGC GCAGCACCAT GGCCTGAAAT 4351 AACCTCTGAA AGAGGAACTT GGTTAGCTAC CTTCTGAGGC GGAAAGAACC 4401 AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 4451 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG 4501 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 4551 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 4601 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT 4651 TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA 4701 AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCGAT 4751 TCTTCTGACA CTAGCGCCAC CATGAAGAAG CCCGAACTCA CCGCTACCAG 4801 CGTTGAAAAA TTTCTCATCG AGAAGTTCGA CAGTGTGAGC GACCTGATGC 4851 AGTTGTCGGA GGGCGAAGAG AGCCGAGCCT TCAGCTTCGA TGTCGGCGGA 4901 CGCGGCTATG TACTGCGGGT GAATAGCTGC GCTGATGGCT TCTACAAAGA 4951 CCGCTACGTG TACCGCCACT TCGCCAGCGC TGCACTACCC ATCCCCGAAG 5001 TGTTGGACAT CGGCGAGTTC AGCGAGAGCC TGACATACTG CATCAGTAGA 5051 CGCGCCCAAG GCGTTACTCT CCAAGACCTC CCCGAAACAG AGCTGCCTGC 5101 TGTGTTACAG CCTGTCGCCG AAGCTATGGA TGCTATTGCC GCCGCCGACC 5151 TCAGTCAAAC CAGCGGCTTC GGCCCATTCG GGCCCCAAGG CATCGGCCAG 5201 TACACAACCT GGCGGGATTT CATTTGCGCC ATTGCTGATC CCCATGTCTA 5251 CCACTGGCAG ACCGTGATGG ACGACACCGT GTCCGCCAGC GTAGCTCAAG 5301 CCCTGGACGA ACTGATGCTG TGGGCCGAAG ACTGTCCCGA GGTGCGCCAC 5351 CTCGTCCATG CCGACTTCGG CAGCAACAAC GTCCTGACCG ACAACGGCCG 5401 CATCACCGCC GTAATCGACT GGTCCGAAGC TATGTTCGGG GACAGTCAGT 5451 ACGAGGTGGC CAACATCTTC TTCTGGCGGC CCTGGCTGGC TTGCATGGAG 5501 CAGCAGACTC GCTACTTCGA GCGCCGGCAT CCCGAGCTGG CCGGCAGCCC 5551 TCGTCTGCGA GCCTACATGC TGCGCATCGG CCTGGATCAG CTCTACCAGA 5601 GCCTCGTGGA CGGCAACTTC GACGATGCTG CCTGGGCTCA AGGCCGCTGC 5651 GATGCCATCG TCCGCAGCGG GGCCGGCACC GTCGGTCGCA CACAAATCGC 5701 TCGCCGGAGC GCAGCCGTAT GGACCGACGG CTGCGTCGAG GTGCTGGCCG 5751 ACAGCGGCAA CCGCCGGCCC AGTACACGAC CGCGCGCTAA GGAGGTAGGT 5801 CGAGTTTAAG ATCTAGAACC GGTCATGGCC GCAATAAAAT ATCTTTATTT 5851 TCATTACATC TGTGTGTTGG TTTTTTGTGT GTTCGAACTA GATGCTGTCG 5901 ACCGATGCCC TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC 5951 GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA 6001 CTCGTAGGAC AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT 6051 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG 6101 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 6151 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 6201 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 6251 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 6301 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 6351 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 6401 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 6451 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 6501 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 6551 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 6601 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 6651 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 6701 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 6751 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 6801 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 6851 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 6901 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 6951 AACTTGGTCT GACAGCGGCC GCAAATGCTA AACCACTGCA File created 26-Oct-09.