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CellTiter-Glo® 3D Cell Viability Assay

A Cell Viability Assay Validated for 3D Microtissue Cultures

  • Accurate 3D cytotoxicity determination
  • Easy assay implementation
  • Simple, 30-minute protocol

Size

Catalog number selected: G9681

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CellTiter-Glo® 3D Cell Viability Assay
10ml
$ 125.00
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CellTiter-Glo-3D_Full-Background

How Does CellTiter-Glo 3D Work?

The CellTiter-Glo® 3D Cell Viability Assay is designed for determining cell viability in 3D microtissue spheroids. The assay reagent penetrates large spheroids and has increased lytic capacity—allowing more accurate determination of viability compared to other assay methods.

Based on the same reliable chemistry as the classic CellTiter-Glo® Assay, this new 3D assay reagent measures ATP as an indicator of viability and generates a luminescent readout that is much more sensitive than colorimetric or fluorescence-based methods. The simple, 30-minute protocol and ready-to-use reagent allows for fast results.

Better ATP Recovery from Larger 3D Microtissue Spheroids

Diameter of Spheroid (μm) Classic CellTiter-Glo® Assay (pmpl/microtissue) Cell-Titer-Glo® 3D Assay (pmpl/microtissue) Ratio
188 16 ± 4 17 ± 4 1.10
386 79 ± 3 94 ± 11 1.19
459 103 ± 2 126 ± 11 1.22
565 127 ± 3 178 ± 17 1.40

Better Lytic Capacity

Improved 3D Microtissue Penetration, More Accurate Viability Data

HCT116 colon cancer spheroids were generated by seeding cells in the InSphero GravityPLUS™ 96-well hanging-drop platform and grown for 4 days.

Panel A. An equivalent volume of reagent was added to all samples, and after 5 minutes of shaking, luminescence was recorded at 30 minutes.

Panel B. A 2X concentration of CellTox™ Green Dye was added to CellTiter-Glo® 3D Reagent (left) or ATPlite™ 1Step Reagent (right) prior to sample addition as an indicator of cell lysis and images were acquired at 30 minutes. The spheroids in Panel B are ~300μm in diameter, and the bars in each image represent a distance of 200μm.

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More Sensitive than Colorimetric or Fluorometric Cell Viability Assays

Luminescent signals from the CellTiter-Glo® 3D Assay are orders of magnitude above background. Other non-lytic viability assays that measure changes in fluorescence (e.g. alamarBlue®) or absorbance (e.g., MTT) generate signals that are only modestly higher than their no-cell control signals.

12331ME_W-a

HCT116 colon cancer cells seeded into an InSphero GravityPLUS™ 96-well hangingdrop platform and grown to generate ~340μm spheroids.

12331ME_W-b

InSphero Insight™ human liver microtissues (~250μm). All microtissues were assayed using the CellTiter-Glo® 3D, alamarBlue® and MTT assays according to manufacturer's protocols. Total assay times for the CellTiter-Glo® 3D, alamarBlue® and MTT assays were 30 minutes, 3 hours and 8 hours, respectively.

Compatible with a Variety of 3D Culture Methods

The results of compound screening using the CellTiter-Glo® 3D Assay in hanging-drop, ultra-low attachment plate (ULA) and Matrigel® 3D cultures are shown below. Equivalent results were achieved for all three methods.

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12386MC-W-b
12386MC-W-c

HCT116 colon cancer cells were seeded as follows: 400 cells in hanging-drop; 1,000 cells in ULA or Matrigel®. Microtissues were grown for 4 days, treated with compounds for 48 hours, and then assayed with the CellTiter-Glo® 3D Reagent. Luminescence was recorded at 30 minutes.

The CellTiter-Glo 3D Assay Demonstrates Excellent Precision

Z'-factor Experiment With 3D Microtissues

Four hundred HCT116 colon cancer cells were seeded into each of 60 wells of a 96-well InSphero GravityPLUS™ hanging-drop plate and incubated for 4 days to form 60 spheroids (~350μm in diameter). Half of the spheroids were treated with 100μM panobinostat (black squares), and the other half were treated with vehicle (1% DMSO, orange squares). After 48 hours, all samples were assayed with the CellTiter-Glo® 3D Reagent. The CellTiter-Glo® 3D Assay provided a Z´-factor of 0.81.

12189MB-W

Specifications

You are viewing: G9681 Change Configuration

What's in the box?

Item Part # Size

CellTiter-Glo® 3D Reagent

G968A 1 × 10ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: G9682 Change Configuration

What's in the box?

Item Part # Size

CellTiter-Glo® 3D Reagent

G968A 10 × 10ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

Specifications

You are viewing: G9683 Change Configuration

What's in the box?

Item Part # Size

CellTiter-Glo® 3D Reagent

G968B 1 × 100ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

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