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Caspase-Glo® 6 Assay Systems

G0970

A Biochemical Assay That Measures Caspase-6 Activity

  • Simple “add-mix-measure" protocol amenable to automation
  • Greater sensitivity than fluorescence-based assays

Size

Catalog number selected: G0970

$ 445.00 Your price: Log In

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Caspase-Glo® 6 Assay Systems
10ml
$ 445.00
Your price: Log In

Caspase-6 is a member of the cysteine aspartic acid-specific protease family and has a key effector role in the cleavage of specific target proteins during apoptosis. The Caspase-Glo® 6 Assay provides a luminogenic substrate, Z-VEID-aminoluciferin, in a buffer optimized for caspase-6 and luciferase activity. The addition of a single Caspase-Glo® 6 Reagent in an add-mix-measure format results in cleavage of the substrate, releasing aminoluciferin, and generation of a glow-type luminescent signal in the presence of Ultra-Glo™ Recombinant Luciferase. The luminescent signal is proportional to the amount of caspase-6 activity present. The homogeneous Caspase-Glo® 6 Assay is designed for use with purified enzyme preparations in multiwell plate formats, making it ideal for automated high-throughput screening for caspase-6 activity and inhibitors of caspase-6 activity.

Features and benefits

  •  Increased accuracy and superior sensitivity over fluorescence-based caspase assays allows inhibitor studies at concentrations below the Km.
  • The maximum signal (and maximum sensitivity) of the assay is reached in as little as 30 minutes after reagent addition.
  • The assay demonstrates an excellent Z´-factor value of 0.86 when using 0.1U/ml of caspase-6 for assays in 384-well plates.
  • The coupled-enzyme, homogeneous format results in a continuous signal, providing excellent stability and allowing plates to be read over an extended period of time.
6728MA.eps
Caspase-6 cleavage of the luminogenic substrate containing the VEID consensus sequence.

Sensitivity Compared to Fluorescent Assay

Determination of IC50 Values

6724MA.eps
Sensitivity of the Caspase-Glo® 6 Assay compared to a fluorescent caspase-6 assay.
6730MA.eps
Determination of IC50 values with the Caspase-Glo® 6 Assay.

Notes

Cat.# G0970 provides sufficient reagents for 100 assays at 100μl/assay or 200 assays at 50μl/assay in 96-well plates or 400 assays at 25μl/assay in 384-well plates.

Protocols

Specifications

What's in the box?

Item Part # Size

Caspase-Glo® 6 Buffer

G095A 1 x 10ml

Z-VEID-Glo™ Substrate

G096A 1 x 100μl

Luciferin Detection Reagent

V859A 1 x 1 each

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

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