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Caspase-Glo® 6 Assay Systems

G0970_Caspase-Glo--6-Assay--10ml_3

We are sorry, but this product is discontinued.

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This product is discontinued
Caspase-Glo® 6 Assay Systems

Caspase-6 is a member of the cysteine aspartic acid-specific protease family and has a key effector role in the cleavage of specific target proteins during apoptosis. The Caspase-Glo® 6 Assay provides a luminogenic substrate, Z-VEID-aminoluciferin, in a buffer optimized for caspase-6 and luciferase activity. The addition of a single Caspase-Glo® 6 Reagent in an add-mix-measure format results in cleavage of the substrate, releasing aminoluciferin, and generation of a glow-type luminescent signal in the presence of Ultra-Glo™ Recombinant Luciferase. The luminescent signal is proportional to the amount of caspase-6 activity present. The homogeneous Caspase-Glo® 6 Assay is designed for use with purified enzyme preparations in multiwell plate formats, making it ideal for automated high-throughput screening for caspase-6 activity and inhibitors of caspase-6 activity.

Features and benefits

  •  Increased accuracy and superior sensitivity over fluorescence-based caspase assays allows inhibitor studies at concentrations below the Km.
  • The maximum signal (and maximum sensitivity) of the assay is reached in as little as 30 minutes after reagent addition.
  • The assay demonstrates an excellent Z´-factor value of 0.86 when using 0.1U/ml of caspase-6 for assays in 384-well plates.
  • The coupled-enzyme, homogeneous format results in a continuous signal, providing excellent stability and allowing plates to be read over an extended period of time.

Caspase-6 cleavage of the luminogenic substrate containing the VEID consensus sequence.

Caspase-6 cleavage of the luminogenic substrate containing the VEID consensus sequence.
Caspase-6 cleavage of the luminogenic substrate containing the VEID consensus sequence.

Sensitivity Compared to Fluorescent Assay

Determination of IC50 Values

Sensitivity of the Caspase-Glo® 6 Assay compared to a fluorescent caspase-6 assay.

Sensitivity of the Caspase-Glo® 6 Assay compared to a fluorescent caspase-6 assay.
Sensitivity of the Caspase-Glo® 6 Assay compared to a fluorescent caspase-6 assay.

Determination of IC50 values with the Caspase-Glo® 6 Assay.

Determination of IC<sub>50</sub> values with the Caspase-Glo® 6 Assay.
Determination of IC50 values with the Caspase-Glo® 6 Assay.

Notes

Cat.# G0970 provides sufficient reagents for 100 assays at 100μl/assay or 200 assays at 50μl/assay in 96-well plates or 400 assays at 25μl/assay in 384-well plates.

Specifications

What's in the box?

Item Part # Size

Caspase-Glo® 6 Buffer

G095A 1 × 10ml

Z-VEID-Glo™ Substrate

G096A 1 × 100μl

Luciferin Detection Reagent

V859A 1 × 1 each

SDS

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Download SDSPDF (90 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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