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Caspase-Glo® 2 Assay Systems

G0940

A Luminescent Assay That Measures Caspase-2 Activity

  • Linear assay can detect activity as low as 0.2 mU/ml
  • High quality assay with superior sensitivity

Size

Catalog number selected: G0940

$ 445.00 Your price: Log In

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Caspase-Glo® 2 Assay Systems
10ml
$ 445.00
Your price: Log In

The Caspase-Glo® 2 Assay is a homogeneous, luminescent assay that measures caspase-2 activity. Caspase-2 is a member of the cysteine aspartic acid-specific protease family. The Caspase-Glo® 2 Assay provides a luminogenic substrate (Z-VDVAD-aminoluciferin) in a reagent optimized for caspase-2 and luciferase activity. A single reagent is added to test samples, resulting in caspase cleavage of the substrate and generation of a glow-type luminescent signal produced by luciferase. Luminescence is proportional to the amount of caspase activity present. The assay system may be used with purified enzyme preparations and is ideal for automated high-throughput screening of inhibitors.

Features and Benefits

Broad Dynamic Range: The assay is linear over four logs of caspase-2 concentration and can detect caspase-2 activity at concentrations as low as 0.2mU/ml.

High-Quality Assay: The assay demonstrates an excellent Z´-factor value of 0.85 in 384-well plates using 0.05U/ml of enzyme.

Increased Accuracy: The superior sensitivity over fluorescence-based caspase assays allows inhibitor studies to be performed below the Km.

Batch Processing Capability: The coupled-enzyme, homogeneous format results in a continuous signal, providing excellent stability and allowing plates to be read over an extended period of time. Luminometers with reagent injectors are not required.

Cat.# G0940 provides sufficient reagents for 100 assays at 100μl/assay or 200 assays at 50μl/assay in 96-well plates or 400 assays at 25μl/assay in 384-well plates. Cat.# G0941 provides sufficient reagents for 500 assays at 100μl/assay or 1,000 assays at 50μl/assay in 96-well plates or 2,000 assays at 25μl/assay in 384-well plates.
6742MA.eps
Sensitivity of the Caspase-Glo® 2 Assay compared to fluorescent assays.

Protocols

Specifications

What's in the box?

Item Part # Size

Caspase-Glo® 2 Buffer

G092A 1 x 10ml

Z-VDVAD-Glo™ Substrate

G093A 1 x 100μl

Luciferin Detection Reagent

V859A 1 x 1 each

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

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