We believe this site might serve you best:

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

CaspACE™ Assay System, Colorimetric

A Complete System for Quantitative Measurement of Caspase-3 Activity

  • A timely assay that measures an early indicator of apoptosis
  • Determines total caspase-3 activity or screens for inducers or inhibitors
  • Use with purified enzyme preparations, cell extracts or tissue lysates

Size

Catalog number selected: G7351

$ 420.00 Your price: Log In

Add to Helix
CaspACE™ Assay System, Colorimetric
50 assays
$ 420.00
Your price: Log In
Change Configuration

The CaspACE™ Assay System, Colorimetric, provides reagents for measuring the activity of caspase-3. The system includes a colorimetric substrate and a cell-permeant inhibitor that allow quantitative measurement of caspase-3 (DEVDase) protease activity. The colorimetric substrate (Ac-DEVD-pNA) provided is labeled with the chromophore p-nitroaniline (pNA). pNA is released from the substrate upon cleavage by DEVDase. Free pNA produces a yellow color that is monitored by a spectrophotometer at 405nm. The amount of yellow color produced upon cleavage is proportional to the amount of DEVDase activity present in the sample.

The potent, irreversible and cell-permeant pan-caspase inhibitor Z-VAD-FMK is provided in the CaspACE™ Assay System, Colorimetric. The addition of the Z-VAD-FMK Inhibitor prior to the induction of apoptosis in cell culture inhibits the activation of the caspase cascade, including caspase-3.

Specifications

You are viewing: G7351 Change Configuration

What's in the box?

Item Part # Size

Caspase Assay Buffer

G595B 1 × 5ml

Caspase Inhibitor Z-VAD-FMK

G723B 1 × 50μl

Caspase-3 Substrate Ac-DEVD-pNA

G724A 1 × 100μl

pNA Standard, 100mM

G725A 1 × 100μl

Cell Lysis Buffer

G726A 1 × 20ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: G7220 Change Configuration

What's in the box?

Item Part # Size

Caspase Assay Buffer

G595B 1 × 5ml

Caspase Inhibitor, Z-VAD-FMK

G723A 1 × 125μl

Caspase-3 Substrate Ac-DEVD-pNA

G724A 2 × 100μl

pNA Standard, 100mM

G725A 1 × 100μl

Cell Lysis Buffer

G726A 1 × 20ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB
Choose your country

Americas

Brazil
Canada
United States

Pacific Asia

Australia
India
Japan
Korea, Republic of
Singapore

Europe

Austria
Belgium
Denmark
Estonia
Finland
France
Germany
Iceland
Italy
Luxembourg
Netherlands
Norway
Poland
Spain
Sweden
Switzerland
United Kingdom