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CaspACE™ Assay System, Colorimetric

A Complete System for Quantitative Measurement of Caspase-3 Activity

  • A timely assay that measures an early indicator of apoptosis
  • Determines total caspase-3 activity or screens for inducers or inhibitors
  • Use with purified enzyme preparations, cell extracts or tissue lysates

Size

Catalog number selected: G7351

$ 420.00 Your price: Log In

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CaspACE™ Assay System, Colorimetric
50 assays
$ 420.00
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The CaspACE™ Assay System, Colorimetric, provides reagents for measuring the activity of caspase-3. The system includes a colorimetric substrate and a cell-permeant inhibitor that allow quantitative measurement of caspase-3 (DEVDase) protease activity. The colorimetric substrate (Ac-DEVD-pNA) provided is labeled with the chromophore p-nitroaniline (pNA). pNA is released from the substrate upon cleavage by DEVDase. Free pNA produces a yellow color that is monitored by a spectrophotometer at 405nm. The amount of yellow color produced upon cleavage is proportional to the amount of DEVDase activity present in the sample.

The potent, irreversible and cell-permeant pan-caspase inhibitor Z-VAD-FMK is provided in the CaspACE™ Assay System, Colorimetric. The addition of the Z-VAD-FMK Inhibitor prior to the induction of apoptosis in cell culture inhibits the activation of the caspase cascade, including caspase-3.

Protocols

Specifications

You are viewing: 50 assays Change Configuration

What's in the box?

Item Part # Size

Caspase Assay Buffer

G595B 1 x 5ml

Caspase Inhibitor Z-VAD-FMK

G723B 1 x 50μl

Caspase-3 Substrate Ac-DEVD-pNA

G724A 1 x 100μl

pNA Standard, 100mM

G725A 1 x 100μl

Cell Lysis Buffer

G726A 1 x 20ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: 100 assays Change Configuration

What's in the box?

Item Part # Size

Caspase Assay Buffer

G595B 1 x 5ml

Caspase Inhibitor, Z-VAD-FMK

G723A 1 x 125μl

Caspase-3 Substrate Ac-DEVD-pNA

G724A 2 x 100μl

pNA Standard, 100mM

G725A 1 x 100μl

Cell Lysis Buffer

G726A 1 x 20ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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