We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Get Up to $500 of Free Promega Reagents with our Back to the Bench Program. Register Now ›

Anti-PARP p85 Fragment pAb

A Specific In Situ Marker of Apoptosis

  • Specifically detects PARP p85 fragment in human, rat and bovine cells and tissues


Catalog number selected: G7341

$ 443.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
Anti-PARP p85 Fragment pAb
$ 443.00
Your price: Log in

Poly (ADP-ribose) polymerase (PARP), a nuclear enzyme involved in DNA repair, is a well known substrate for caspase-3 cleavage during apoptosis. Anti-PARP p85 Fragment pAb is a rabbit polyclonal antibody specific for the p85 fragment of PARP in human, rat and bovine cells and tissues that results from caspase cleavage of the 116kDa intact molecule. The rabbit polyclonal antibody is affinity-purified using a peptide that corresponds to a region of the p85 fragment of PARP. The PARP immunogen is a synthetic peptide, gly-val-asp-glu-val-ala-lys (GVDEVAK), representing the N-terminus of the large C-terminal fragment of human PARP that results from caspase-3 cleavage. Each batch of antibody is quality assurance tested for use in immunostaining applications and contains sufficient antibody for 50 immunocytochemical reactions at the suggested working dilution of 1:100.

Other suggested working dilutions:

  • Western blotting (1:300 to 1:1,000)
  • Immunohistochemistry (1:50)
  • Flow cytometry

For more information, see the Protocols & Applications Guide.

Anti-PARP p85 Fragment pAb and TUNEL double-labeling of apoptotic Jurkat cells.

Anti-PARP p85 Fragment pAb and TUNEL double-labeling of apoptotic Jurkat cells.
Anti-PARP p85 Fragment pAb and TUNEL double-labeling of apoptotic Jurkat cells.
Cells were labeled with the Anti-PARP p85 Fragment pAb (red) and the DeadEnd™ Fluorometric TUNEL System (Cat.# G3250; green). The colocalization of cleaved PARP in cells containing TUNEL-positive nuclei demonstrates that the Anti-PARP p85 Fragment pAb specifically labels apoptotic cells. Protocols developed and performed at Promega.


What's in the box?

Item Part # Size

Anti-PARP p85 Fragment pAb

G734A 1 × 50μl


Choose language:

Download SDSPDF (192 KB) – English (United States)

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


Patents and Disclaimers

U.S. Pat. Nos. 6,350,452 and 7,037,664.

Let's find the product that meets your needs.

Talk to a Scientist