We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Anti-PARP p85 Fragment pAb

A Specific In Situ Marker of Apoptosis

  • Specifically detects PARP p85 fragment in human, rat and bovine cells and tissues

Size

Catalog number selected: G7341

$ 415.00 Your price: Log In

Add to Helix
Anti-PARP p85 Fragment pAb
50μl
$ 415.00
Your price: Log In

Poly (ADP-ribose) polymerase (PARP), a nuclear enzyme involved in DNA repair, is a well known substrate for caspase-3 cleavage during apoptosis. Anti-PARP p85 Fragment pAb is a rabbit polyclonal antibody specific for the p85 fragment of PARP in human, rat and bovine cells and tissues that results from caspase cleavage of the 116kDa intact molecule. The rabbit polyclonal antibody is affinity-purified using a peptide that corresponds to a region of the p85 fragment of PARP. The PARP immunogen is a synthetic peptide, gly-val-asp-glu-val-ala-lys (GVDEVAK), representing the N-terminus of the large C-terminal fragment of human PARP that results from caspase-3 cleavage. Each batch of antibody is quality assurance tested for use in immunostaining applications and contains sufficient antibody for 50 immunocytochemical reactions at the suggested working dilution of 1:100.

Other suggested working dilutions:

  • Western blotting (1:300 to 1:1,000)
  • Immunohistochemistry (1:50)
  • Flow cytometry

For more information, see the Protocols & Applications Guide.

2734CA08_9A.eps
Anti-PARP p85 Fragment pAb and TUNEL double-labeling of apoptotic Jurkat cells.
Cells were labeled with the Anti-PARP p85 Fragment pAb (red) and the DeadEnd™ Fluorometric TUNEL System (Cat.# G3250; green). The colocalization of cleaved PARP in cells containing TUNEL-positive nuclei demonstrates that the Anti-PARP p85 Fragment pAb specifically labels apoptotic cells. Protocols developed and performed at Promega.

Protocols

Specifications

What's in the box?

Item Part # Size

Anti-PARP p85 Fragment pAb

G734A 1 x 50μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,350,452 and 7,037,664.

Resources

Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom