Poly (ADP-ribose) polymerase (PARP), a nuclear enzyme involved in DNA repair, is a well known substrate for caspase-3 cleavage during apoptosis. Anti-PARP p85 Fragment pAb is a rabbit polyclonal antibody specific for the p85 fragment of PARP in human, rat and bovine cells and tissues that results from caspase cleavage of the 116kDa intact molecule. The rabbit polyclonal antibody is affinity-purified using a peptide that corresponds to a region of the p85 fragment of PARP. The PARP immunogen is a synthetic peptide, gly-val-asp-glu-val-ala-lys (GVDEVAK), representing the N-terminus of the large C-terminal fragment of human PARP that results from caspase-3 cleavage. Each batch of antibody is quality assurance tested for use in immunostaining applications and contains sufficient antibody for 50 immunocytochemical reactions at the suggested working dilution of 1:100.
Other suggested working dilutions:
- Western blotting (1:300 to 1:1,000)
- Immunohistochemistry (1:50)
- Flow cytometry
For more information, see the Protocols & Applications Guide.