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UGT-Glo™ Assay

V2081

A Luminescent Method for Measuring UGT Activity from a Variety of Sources

  • Luminescent format eliminates need for time-consuming analyses
  • Simple and sensitive protocol saves time and money

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Catalog number selected: V2081

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UGT-Glo™ Assay
Assay system/200 assays
$ 330.00
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The UGT-Glo™ Assay provides a luminescent method for measuring UDP glucuronosyltransferase (UGT) activity. The UGT-Glo™ Assay is designed to measure UGT activity from a variety of sources, such as microsomes containing recombinantly expressed enzymes or microsomal preparations derived from mammalian tissues, and to test the effects of various chemicals on UGT activity.

The assay involves incubating UGT with a proluciferin substrate; a portion of the substrate gets conjugated with UDP, while the remainder is unmodified. Upon the addition of d-Cysteine, the unconjugated proluciferin is converted into luciferin and, in a coupled reaction with luciferase/luciferin, is converted into light. Conjugated proluciferin remains intact and does not contribute to the luminescence. Thus, the signal generated is inversely correlated with UGT activity present in the sample.

The UGT-Glo™ Assay contains two proluciferin substrates: the UGT Multienzyme Substrate, which is compatible with a wide range of UGTs, and the UGT1A4 Substrate, which reacts specifically with UGT1A4. The kit also contains Luciferin Detection Reagent and Reconstitution Buffer, UGT Buffer, d-Cysteine and UDPGA.


Conversion of UGT Multienzyme Substrate

7984MA.eps
Conversion of UGT Multienzyme Substrate by UGT enzymes.

 

 

UGT enzymes attach a glucuronic acid moiety to the proluciferin substrate. During the detection step, the unconjugated proluciferin is converted to luciferin by cyclization with D-cysteine and then converted into light by luciferase. Glucuronidated proluciferin does not get converted into luciferin, and thus does not produce light. Light output is inversely proportional to UGT enzymatic activity.

Features and Benefits

  • The luminescent format eliminates the need for time-consuming analyses such as HPLC and LC/MS.
  • The simple "add and read" protocol makes the assay amenable to higher throughput screening in multiwell plates.
  • Use less enzyme and scale down reaction volumes; save on reagent costs.

Applications include screening drugs and new chemical entities for their capacity to modulate UGT activity in native or recombinant fractions and measuring recombinant UGT activities in membrane fractions from heterologous expression systems, such as insect cells and E. coli.

Protocols

Specifications

You are viewing: 200 assays Change Configuration

What's in the box?

Item Part # Size

UDPGA, 50mM

V209A 1 x 640μl

UGT Buffer, 5X

V210A 1 x 1.6ml

UGT Multienzyme Substrate

V213A 1 x 10μl

d-Cysteine, 100X

V251A 1 x 100μl

UGT1A4 Substrate

V260A 1 x 10μl

Luciferin Detection Reagent

V859A 1 x 1 each

Reconstitution Buffer

V865A 1 x 10ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Store UGT enzymes and control membranes at –70°C. Store remaining components at –20°C.

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 1,000 assays Change Configuration

What's in the box?

Item Part # Size

UDPGA, 50mM

V209B 1 x 3.2ml

UGT Buffer, 5X

V210B 1 x 8ml

UGT Multienzyme Substrate

V213B 1 x 50μl

d-Cysteine, 100X

V251B 1 x 500μl

UGT1A4 Substrate

V260B 1 x 50μl

Luciferin Detection Reagent

V859B 1 x 1 each

Reconstitution Buffer

V865B 1 x 50ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Store UGT enzymes and control membranes at –70°C. Store remaining components at –20°C.

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 1,000 assays Change Configuration

What's in the box?

Item Part # SizeConcentration

UDPGA, 50mM

V209B 1 x 3.2ml

UGT Buffer, 5X

V210B 1 x 8ml

UGT Multienzyme Substrate

V213B 1 x 50μl

UGT1A1 Microsomes

V241B 1 x 800μl

d-Cysteine, 100X

V251B 1 x 500μl

UGT1A4 Substrate

V260B 1 x 50μl

Control Membranes

V476A 1 x 100μl5mg/ml

Luciferin Detection Reagent

V859B 1 x 1 each

Reconstitution Buffer

V865B 1 x 50ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Store UGT enzymes and control membranes at –70°C. Store remaining components at –20°C.

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 1,000 assays Change Configuration

What's in the box?

Item Part # SizeConcentration

UDPGA, 50mM

V209B 1 x 3.2ml

UGT Buffer, 5X

V210B 1 x 8ml

UGT Multienzyme Substrate

V213B 1 x 50μl

UGT2B7 Microsomes

V245B 1 x 800μl

d-Cysteine, 100X

V251B 1 x 500μl

UGT1A4 Substrate

V260B 1 x 50μl

Control Membranes

V476A 1 x 100μl5mg/ml

Luciferin Detection Reagent

V859B 1 x 1 each

Reconstitution Buffer

V865B 1 x 50ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Store UGT enzymes and control membranes at –70°C. Store remaining components at –20°C.

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Resources

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Small Molecule Drug Discovery Workflow

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