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P450-Glo™ CYP3A7 Assay System

A Luminescent Method for Measuring CYP3A7 Activity

  • Luminescent format eliminates need for time-consuming analyses
  • Broad dynamic range and low background

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Catalog number selected: V8811

$ 145.00
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P450-Glo™ CYP3A7 Assay System
10ml
$ 145.00
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The P450-Glo™ CYP3A7 Assay provides a homogeneous, luminescent method for measuring cytochrome P450 CYP3A7 activity. The assay employs luminogenic P450 substrates that are derivatives of beetle luciferin, a substrate for luciferase enzymes. The derivatives are not substrates for luciferase but are converted by P450s to luciferin, which in turn reacts with luciferase to produce light that is directly proportional to the activity of the P450. 

This luminescent assay exhibits exquisite sensitivity, low background signals and broad dynamic range. It generates a "glow-type" luminescent signal, produced using derivatized luciferins as P450 substrates and a recombinant stabilized luciferase (Ultra-Glo Luciferase) coupled with a proprietary buffer system. The half-life of the luminescent output is greater than two hours, eliminating the need for luminometers with injectors and allowing batch plate processing. The luminescent format also eliminates the need for time-consuming analyses such as HPLC.

The luciferin-based substrates are readily taken up by cells and rapidly converted into luciferin inside the cell, which reduces the incubation time required (typically 30-60 minutes). Other benefits of the P450-Glo™ CYP3A7 Assay include a formulation that minimizes the incidence of false positives due to inhibition of luciferase by analytes when screening for cytochrome P450 inhibitors. Z´ values greater than 0.8 are achieved in either 96- or 384-well plate formats and confer highly predictive results.

 Assays are complete systems that include a membrane preparation containing recombinant human cytochrome P450 enzyme, a luminogenic cytochrome P450 substrate appropriate for the enzyme, an NADPH regeneration system, reaction buffer, Luciferin Detection Reagent and Luciferin-Free Water.

Specifications

You are viewing: V8811 Change Configuration

What's in the box?

Item Part # Size

Luciferin Detection Reagent

V859A 1 × 1 each

Reconstitution Buffer

V865A 1 × 10ml

Luciferin-BE, 5mM

V866C 1 × 300μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.

Specifications

You are viewing: V8812 Change Configuration

What's in the box?

Item Part # Size

Luciferin Detection Reagent

V859B 1 × 1 each

Reconstitution Buffer

V865B 1 × 50ml

Luciferin-BE, 5mM

V866D 2 × 750μl

SDS

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Certificate of Analysis

See all certificates for V8812 Search
View more results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.

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