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P450-Glo CYP3A4 Assay and Screening System

V9920

Luminescent Assays and Screening Systems for Measuring CYP3A4 Activity

  • Screening systems include complete set of reagents
  • Luminescent format eliminates need for time-consuming analyses
  • Broad dynamic range and low background

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Choose a luciferin substrate

Catalog number selected: V8801

$ 140.00 Your price: Log In

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P450-Glo CYP3A4 Assay and Screening System
10ml Assay
$ 140.00
Your price: Log In
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The P450-Glo™ CYP3A4 Assays provide a homogeneous, luminescent method for measuring cytochrome P450 CYP3A4 activity. The assays are designed to measure the activities of P450s from recombinant and native sources and for testing the effects of analytes such as drugs and new chemical entities on P450 activities. These luminescent assays exhibit exquisite sensitivity, low background signals and broad dynamic range.

P450-Glo™ Assays employ luminogenic P450 substrates that are derivatives of beetle luciferin, a substrate for luciferase enzymes. The derivatives are not substrates for luciferase but are converted by P450s to luciferin, which in turn reacts with luciferase to produce light that is directly proportional to the activity of the P450.

The P450-Glo™ Assays generate a "glow-type" luminescent signal, produced using derivatized luciferins as P450 substrates and a recombinant stabilized luciferase (Ultra-Glo™ Luciferase) coupled with a proprietary buffer system. The half-life of the luminescent output is greater than two hours, eliminating the need for luminometers with injectors and allowing batch plate processing. The formulation also minimizes the incidence of false positives due to inhibition of luciferase by analytes when screening for cytochrome P450 inhibitors.

Dimethyl sulfoxide (DMSO), a common solvent used to solubilize chemical compounds, can significantly inhibit the activity of the 3A4 isoform of cytochrome P450, even at low concentrations (<0.1%).The P450-Glo™ CYP3A4 System (Luciferin-PPXE) DMSO-Tolerant Assay is specifically designed to tolerate DMSO in the 3A4 reaction. The assay exhibits little to no change in the signal-to-background ratio in the presence of 0.2% DMSO compared to a no-DMSO control.

Simple Protocol; Short Incubation Time

7981MA

A variety of CYP3A4 systems to fit your research needs

P450-Glo™ CYP3A4 Assay and Screening System

P450-Glo™ CYP3A4 Assay and Screening System with Luciferin-IPA

P450-Glo™ CYP3A4 Assay and Screening System (Luciferin-PPXE) DMSO-Tolerant

P450-Glo™ CYP3A4 Assay (Luciferin-PFBE) Cell-Based/Biochemical Assay

 

Protocols

Specifications

You are viewing: V8801 Change Configuration

What's in the box?

Item Part # Size

Luciferin Detection Reagent

V859A 1 × 1 each

Reconstitution Buffer

V865A 1 × 10ml

Luciferin-BE, 5mM

V866A 1 × 100μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: V8802 Change Configuration

What's in the box?

Item Part # Size

Luciferin Detection Reagent

V859B 1 × 1 each

Reconstitution Buffer

V865B 1 × 50ml

Luciferin-BE, 5mM

V866B 1 × 500μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: V9920 Change Configuration

What's in the box?

Item Part # SizeConcentration

Reconstitution Buffer with Esterase

V144B 1 × 50ml

Luciferin-Free Water

V348A 1 × 50ml

Potassium Phosphate Buffer, 1M

V446A 1 × 5ml

Control Membranes

V476A 1 × 100μl5mg/ml

CYP3A4 (1pmol/μl) + Reductase + b5

V480B 1 × 100μl

Luciferin-IPA

V840B 1 × 60μl

Luciferin Detection Reagent

V859B 1 × 1 each

Solution A—NADPH Regeneration System

V952A 1 × 2.75ml

Solution B—NADPH Regeneration System

V953A 1 × 600μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

U.S. Pat. No. 8,288,559 and other patents pending.

Specifications

You are viewing: V9910 Change Configuration

What's in the box?

Item Part # SizeConcentration

Luciferin-Free Water

V348A 1 × 50ml

2M Tris-HCl (pH 7.5), 20X

V349A 1 × 1ml

Potassium Phosphate Buffer, 1M

V446A 2 × 5ml

Control Membranes

V476A 1 × 100μl5mg/ml

CYP3A4 (1pmol/μl) + Reductase + b5

V480A 1 × 500μl

Luciferin Detection Reagent

V859B 1 × 1 each

Reconstitution Buffer

V865B 1 × 50ml

Solution A—NADPH Regeneration System

V952A 1 × 2.75ml

Solution B—NADPH Regeneration System

V953A 1 × 600μl

Luciferin-PPXE

V997A 2 × 15μl50mM

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

U.S. Pat. No. 8,288,559 and other patents pending.

U.S. Pat. No. 8,592,172 and other patents pending.

U.S. Pat. No. 8,551,721 and other patents pending.

Specifications

You are viewing: V9800 Change Configuration

What's in the box?

Item Part # SizeConcentration

Luciferin-Free Water

V348A 1 × 50ml

Potassium Phosphate Buffer, 1M

V446A 2 × 5ml

Control Membranes

V476A 1 × 100μl5mg/ml

CYP3A4 (1pmol/μl) + Reductase + b5

V480A 2 × 500μl

Luciferin Detection Reagent

V859B 1 × 1 each

Reconstitution Buffer

V865B 1 × 50ml

Luciferin-BE, 5mM

V866B 1 × 500μl

Solution A—NADPH Regeneration System

V952A 1 × 2.75ml

Solution B—NADPH Regeneration System

V953A 1 × 600μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

U.S. Pat. No. 8,288,559 and other patents pending.

U.S. Pat. No. 8,592,172 and other patents pending.

U.S. Pat. No. 8,551,721 and other patents pending.

Specifications

You are viewing: V9001 Change Configuration

What's in the box?

Item Part # Size

Reconstitution Buffer with Esterase

V144A 1 × 10ml

Luciferin-IPA

V840A 1 × 15μl

Luciferin Detection Reagent

V859A 1 × 1 each

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

U.S. Pat. No. 8,288,559 and other patents pending.

Specifications

You are viewing: V8911 Change Configuration

What's in the box?

Item Part # SizeConcentration

Luciferin Detection Reagent

V859A 1 × 1 each

Reconstitution Buffer

V865A 1 × 10ml

Luciferin-PPXE

V997A 1 × 15μl50mM

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

U.S. Pat. No. 8,288,559 and other patents pending.

U.S. Pat. No. 8,592,172 and other patents pending.

U.S. Pat. No. 8,551,721 and other patents pending.

Specifications

You are viewing: V8912 Change Configuration

What's in the box?

Item Part # SizeConcentration

Luciferin Detection Reagent

V859B 1 × 1 each

Reconstitution Buffer

V865B 1 × 50ml

Luciferin-PPXE

V997A 2 × 15μl50mM

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

U.S. Pat. No. 8,288,559 and other patents pending.

U.S. Pat. No. 8,592,172 and other patents pending.

U.S. Pat. No. 8,551,721 and other patents pending.

Specifications

You are viewing: V8901 Change Configuration

What's in the box?

Item Part # SizeConcentration

Luciferin Detection Reagent

V859A 1 × 1 each

Reconstitution Buffer

V865A 1 × 10ml

Luciferin-PFBE

V873A 1 × 500μl2mM

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

U.S. Pat. No. 8,288,559 and other patents pending.

U.S. Pat. No. 8,592,172 and other patents pending.

U.S. Pat. No. 8,551,721 and other patents pending.

Specifications

You are viewing: V8902 Change Configuration

What's in the box?

Item Part # SizeConcentration

Luciferin Detection Reagent

V859B 1 × 1 each

Reconstitution Buffer

V865B 1 × 50ml

Luciferin-PFBE

V873A 3 × 500μl2mM

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

U.S. Pat. No. 8,288,559 and other patents pending.

U.S. Pat. No. 8,592,172 and other patents pending.

U.S. Pat. No. 8,551,721 and other patents pending.

Specifications

You are viewing: V9002 Change Configuration

What's in the box?

Item Part # Size

Reconstitution Buffer with Esterase

V144B 1 × 50ml

Luciferin-IPA

V840B 1 × 60μl

Luciferin Detection Reagent

V859B 1 × 1 each

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

U.S. Pat. No. 8,288,559 and other patents pending.

Resources

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