Human Vitronectin is purified from plasma. The plasma was tested and found negative for HIV-1 antibody and Hepatitis B antigen.
Vitronectin belongs to the group of structurally and functionally homologous adhesive proteins (fibrinogen, fibronectin, Von Willebrand factor) that interact with platelets and the vessel wall in the early stages of blood clotting. When coated on surfaces, very low concentrations of Vitronectin promote endothelial cell attachment and induce spreading and migration of cells in a time- and concentration-dependent fashion.
Activity: When coated onto tissue culture plastic, Vitronectin promotes one-half maximal attachment of BALB/3T3 fibroblasts in serum-free medium below 0.1μg/cm2. Maximal attachment occurs at approximately 0.2μg/cm2.
- Preissner, K.E. (1989) Blut 59, 419–31.