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T7 RiboMAX™ Express Large Scale RNA Production System

Produces 5–8.5mg/ml RNA in 30 Minutes

  • Consistent production of milligram amounts of RNA
  • Minimal pipetting steps and setup time
  • Efficiently transcribes DNA templates of varying sizes. Works with transcripts as short as 21bp.
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Catalog number selected: P1320

$ 413.00
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T7 RiboMAX™ Express Large Scale RNA Production System
1 system
$ 413.00
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Quickly Produce Milligram Amounts of RNA

The T7 RiboMAX™ Express Large Scale RNA Production System quickly generates large quantities of uncapped RNA transcripts suitable for in vitro or in vivo translation. RNA synthesized with the T7 RiboMAX™ Express Large Scale RNA Production System performs better for in vitro rabbit reticulocyte translation systems than RNA synthesized by standard methods. This is especially notable with high RNA concentrations that normally inhibit in vitro translation. 

The T7 RiboMAX™ Express Large Scale RNA Production System offers a setup with minimal pipetting steps to increase efficiency and reduce potential errors. In order to provide this easy setup, a 2X transcription buffer that already includes all four rNTPs is included. The system also provides RQ1 RNase-Free DNase for the degradation of plasmid template after transcription.

Due to the combined 2X buffer and rNTPs, the T7 RiboMAX™ Express System is not recommended for the synthesis of RNA for applications that require capped RNA. For synthesis of capped RNA, please order the standard RiboMAX™ Large Scale RNA Production System—T7 (Cat.# P1300).

Applications of the T7 RiboMAX™ Express System include:

  • Creation of molecular standards for RT-qPCR assays
  • Generation of dsRNA and long RNAs for nonmammalian RNA interference studies
  • In vitro synthesis of tRNA and rRNA, and RNA virus genomes and ribozymes
  • Production of substrates for RNA splicing, studies of RNA secondary structure and RNA:protein interactions
  • Synthesis of antisense RNA
RNA yield from RiboMax Express RNA production system

RNA yield. The RNA yield from T7 RiboMax™ Express in vitro transcription reactions incubated for the indicated time periods was determined by UV absorbance at 260nm. The numbers on top of each bar represent the RNA yield in micrograms resulting from in vitro transcription reactions with a volume of 20µl.

Analysis of in vitro transcripts by agarose gel electrophoresis.
Analysis of T7 RiboMax™ Express-generated in vitro transcripts. In vitro transcribed RNAs were visualized by agarose (1%) gel electrophoresis in 1X TBE/0.1% SDS buffer and subsequent ethidium bromide staining. Each lane contains 1μg of RNA resulting from in vitro transcription reactions incubated for the indicated time periods. Full-length RNA of 27.3kb is indicated. DNA fragment sizes of 500ng of EcoRI- and HindIII-digested bacteriophage lambda DNA (lane M) are shown.

Specifications

What's in the box?

Item Part # SizeAvailable Separately

RQ1 RNase-Free DNase

M610C 1 × 110u View Product

Nuclease-Free Water

P119A 1 × 1,250μl View Product

Enzyme Mix, T7 Express

P132A 1 × 100μl

Sodium Acetate, 3.0M (pH 5.2)

P135A 1 × 1ml

RiboMAX™ Express T7 2X Buffer

P164A 1 × 500μl

pGEM® Express Positive Control Template

P256A 2 × 5μg View Product

SDS

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Download SDSPDF (221 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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