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Save 20% on ReliaPrep™️ DNA & RNA Clean-Up and Concentration Systems through December 31st. Shop Now › 

RiboMAX™ Large Scale RNA Production Systems

Capped or Uncapped RNA Transcripts for in vitro or in vivo Translation

  • Produces 2–5mg/ml RNA in a 1ml reaction
  • Available for use with SP6 or T7 RNA polymerase
  • Scalable to meet varying RNA production requirements
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Catalog number selected: P1280

$ 328.00
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RiboMAX™ Large Scale RNA Production Systems
1 system
$ 328.00
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More RNA from Transcription Reactions

The RiboMAX™ Large Scale RNA Production System generates large quantities of capped or uncapped RNA transcripts suitable for in vitro or in vivo translation. RNA synthesized with RiboMAX™ Large Scale RNA Production System performs better for in vitro rabbit reticulocyte translation systems than RNA synthesized by standard methods. This is especially notable with high RNA concentrations that normally inhibit in vitro translation. With the reduction of components inhibitory to translation, there may be an advantage for other applications requiring biologically active RNA.

The RiboMAX™ Large Scale RNA Production System consistently produces about 10- to 20-fold more RNA than is produced with the standard Riboprobe® System. Because the RiboMAX™ Systems produce such large quantities of RNA, they are not recommended for generation of high-specific-activity RNA probes, due to the cost requirement of radiolabeled nucleotides. 

The RiboMAX™ System reactions differ from those of the Riboprobe® Systems in three main ways: a HEPES (pH 7.5) buffer is used rather than a Tris-HCl (pH 7.9) buffer; rNTP and magnesium concentrations are elevated at levels appropriate for either SP6 or T7 RNA polymerase; and inorganic pyrophosphatase is included in the reaction.

Note: Use of the RiboMAX™ System for production of capped transcripts requires separate purchase of the Ribo m7G Cap Analog (Cat. P1711).

Applications of the RiboMAX™ Systems include:

  • Generation of dsRNA and long RNAs for nonmammalian RNA interference studies.
  • In vitro synthesis of tRNA and rRNA, and RNA virus genomes and ribozymes.
  • Production of substrates for RNA splicing, studies of RNA secondary structure and RNA:protein interactions.
  • Synthesis of antisense RNA

References

  1. Gurevich, V.V. et al. (1991) Anal. Biochem. 195, 207–13.
  2. Beckler, G.S. (1992) Promega Notes 39, 12–6.
RiboMax produces more RNA than standard in vitro transcription protocols.

Effect of pGEM-luc RNA concentration on in vitro translation. The indicated amounts of pGEM-luc RNA transcripts were used to program 50uL Rabbit Reticulocyte Lysate in vitro translation reactions. Expression of functional luciferase was measured as follows: 2.5µl  of each reaction was mixed with an equal volume of Luciferase Assay Reagent and luminescence  measured for 10 seconds in a luminometer.

Specifications

You are viewing: P1280 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

rATP, 100mM

E601D 1 × 100μl100mM View Product

rUTP, 100mM

E602D 1 × 100μl100mM View Product

rGTP, 100mM

E603D 1 × 100μl100mM View Product

rCTP, 100mM

E604D 1 × 100μl100mM View Product

SP6 Transcription Buffer, 5X

P131A 1 × 240μl

SP6 Linear Control DNA

P134A 1 × 10μl

Sodium Acetate, 3.0M (pH 5.2)

P135A 1 × 1ml

Enzyme Mix, SP6 RNA Polymerase

P133A 1 × 120μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product

RQ1 RNase-Free DNase

M610C 1 × 110u View Product

SDS

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Download SDSPDF (260 KB) – English (United States)

Certificate of Analysis

See all certificates for P1280

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: P1300 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

RQ1 RNase-Free DNase

M610C 1 × 110u View Product

rATP, 100mM

E601D 1 × 100μl100mM View Product

rUTP, 100mM

E602D 1 × 100μl100mM View Product

rGTP, 100mM

E603D 1 × 100μl100mM View Product

rCTP, 100mM

E604D 1 × 100μl100mM View Product

Nuclease-Free Water

P119A 1 × 1,250μl View Product

Sodium Acetate, 3.0M (pH 5.2)

P135A 1 × 1ml

Enzyme Mix, T7 RNA Polymerase

P137A 1 × 120μl

T7 Transcription Buffer, 5X

P140A 1 × 240μl5X

T7 Linear Control DNA

P141A 1 × 10μl

SDS

Choose language:

Download SDSPDF (256 KB) – English (United States)

Certificate of Analysis

See all certificates for P1300

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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