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Maximize Your Reverse Transcription-qPCR (RT-qPCR) Assays

Carl was an academic researcher for 15 years before joining Promega in 2005. Carl earned his Ph.D. from the University of Virginia, and also enjoyed time as a researcher at Texas A&M University, The Scripps Research Institute and the Medical College of Wisconsin.

He has worked in both prokaryotic & eukaryotic systems, including bacteria, plant, cell culture and animal models. The two unifying theme of his research was the elucidation of the molecular mechanisms of circadian rhythms. He has wide experience in molecular and cell-based techniques, including extensive experience with real-time PCR. Carl continues to focus on real-time PCR in his technical support role at Promega.

  • Carl Strayer

  • Ph.D.

  • Original Webinar Date: Tuesday, February 14, 2012 Tuesday, February 14, 2012

ABSTRACT

RT-qPCR assays, the workhorse of most gene expression studies, may be daunting to the novice user given the use of sophisticated instruments, expensive reagents, and the apparent complexity of the technique, and experienced users may find it challenging to develop RT-qPCR assays that are both sensitive and highly reproducible. Yet underneath the surface, RT-qPCR is both simple and extremely powerful when carefully implemented.

This webinar aims to both educate novice users to appreciate the capabilities of this assay and empower them to implement it in their research. We cover the basics of RT-qPCR including an overview of the technique and options available, as well as data analysis and interpretation. For the more experienced user, we provide in depth coverage of common, but often misunderstood, problems and their solutions. Real-world examples of gene expression studies using this powerful technique are also presented.

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