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Promega Webinars

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. Most presentations are given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

Post-Translational Modification Enzymes: How to Interrogate Key Drug Targets

Cell-Based Assays

Tuesday, April 11, 2017

Hicham Zegzouti, PhD

Post-translational modifications (PTMs), the addition of functional groups to proteins, play a significant role in regulating cellular biology. Examples include phosphorylation, a common mechanism in regulating enzymatic activity, and the addition of carbohydrates to many eukaryotic proteins, which promotes proper folding, improves stability and serves in regulatory functions. A key objective of research and drug discovery surrounding PTMs is to study the regulation of these enzymes and find specific modulators of their activity. Although mass spectrometry, eastern and western blotting detection technologies have been used traditionally, none are amenable to high throughput or as sensitive as bioluminescent detection of nucleotide-based substrates for such enzymes.

How to Avoid Artificial Non-Enzymatic PTMs During the Peptide Mapping Sample Preparation

Proteomics

Tuesday, April 25, 2017

Sergei Saveliev, PhD

Non-enzymatic post-translational modifications (PTMs) spontaneously occur in biotherapeutic proteins during manufacturing and storage. These modifications negatively affect efficacy and stability of biotherapeutic proteins. Major non-enzymatic PTMs are deamidation, disulfide bond scrambling and oxidation. These non-enzymatic PTMs are also introduced during protein preparation for peptide mapping and compromise the analysis. In this webinar we will discuss sources of these artificial protein modifications as well as procedural optimizations to suppress these PTMs.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

Results from a Casework Pilot Study for PowerPlex® Y23 System

Genetic Identity

Monday, December 19, 2016

Mr. Jim Thomson

The PowerPlex® Y23 System is a 23-loci, 5-color Y-STR multiplex designed for genotyping forensic casework samples, database samples and paternity samples. In this webinar, Jim Thomson from LGC Group described the use of PowerPlex® Y23 System on difficult sexual offense cases, particularly those in which no sperm was identified. He shared success rates on different categories derived from a case study carried out on samples provided by nominated participating UK police forces.

Guidelines for Developing Robust and Reliable PCR Assays

MDx

Tuesday, December 06, 2016

Leta Steffen, PhD

Don't settle for a mediocre PCR assay. Learn how experts develop robust and reliably performing PCR assays.

The Key Role of FcR Activating Antibodies in Generating "Sterilizing" Immunity Against Herpes Simplex Virus

Drug Discovery

Tuesday, November 08, 2016

Betsy Herold, MD

Antibody dependent cell mediated cytotoxicity (ADCC) is an important immune effector mechanism against tumor and virus-infected cells. In recent years, ADCC has emerged as an important protective immune correlate in a number of vaccine studies for HIV and influenza. This webinar will highlight recent studies demonstrating the central role of FcR functions in vaccine-mediated protection against HSV-1 and HSV-2 infection, challenging the existing paradigm that high titer neutralizing antibodies are required.

Faster Mass Spec: Same-Day Sample Prep Now a Reality

Proteomics

Tuesday, October 25, 2016

Micheal M. Rosenblatt, PhD

Traditional protocols require digestion times of up to 18 hours, as well as reduction and alkylation prior to digestion. These denaturants often inhibit enzymes and modify proteins, and require removal prior to analysis. What if you could shorten digestion times to ~30 minutes, without denaturants or reductants or alkylating agents?

Immune Checkpoint Bioassays Power Combination Immunotherapy

Drug Discovery

Tuesday, September 27, 2016

Richard Somberg, PhD

Therapeutic antibodies designed to block or activate immune checkpoint receptors are a powerful new approach to the treatment of cancer and other diseases. However, due to their biologic complexity the development of therapeutic antibodies requires unique functional and analytical tools. Here we present a suite of MOA-based functional bioassays for use in antibody screening, characterization, potency testing and stability studies.

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