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Promega Webinars

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. Most presentations are given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

Metagenomic Mapping of Medical, Urban and Space Environments

MDx

Tuesday, December 05, 2017

Christopher Mason, PhD

Many new methods in genomics enable an integrative, cross-kingdom view of patients (precision metagenomics) and their environment, including metagenome profiles of the world’s cities (MetaSUB.org) and antimicrobial resistance (AMR) markers. In this webinar, technologies that can sequence, quantify, and map nucleic acids will be covered, for Earth and beyond, focusing on methods from Promega that enable these projects and missions.

When Medical Conditions Muddle Forensic Casework

Genomics

Friday, December 08, 2017

Dr. Ashley Anderson

While no case is without its challenges, medical conditions such as chimerism and genetic outliers (e.g., identical twins) add wrinkles to the analysis and interpretation of the DNA profiles obtained in the case. Medical procedures such as bone marrow transplants can also confuse the results, affecting the legal, social and ethical deliberations of the case. In this webinar, Dr. Anderson will review the interpretation and reporting difficulties experienced when medical conditions have the potential to influence forensic casework results.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

How to Integrate Cellular Metabolism Assays Into Your Research: Considerations and Challenges

Cell-Based Assays

Tuesday, September 12, 2017

Donna Leippe, PhD

Metabolite detection technologies can pose challenges such as laborious sample preparation and the inability to assay numerous samples rapidly. Additionally, lack of sensitivity and dynamic range prevents detection of metabolites directly in cells in multi-well plates. In this webinar, we describe new bioluminescent metabolite assays and sample processing strategies that enable rapid measurements of key energy metabolites in plate formats and show how the assays can be used to study cancer cell metabolism and activation of T cells.

How to Avoid Artificial Non-Enzymatic PTMs During the Peptide Sample Preparation Process

Proteomics

Tuesday, June 13, 2017

Sergei Saveliev, PhD

Non-enzymatic post-translational modifications (PTMs) spontaneously occur in biotherapeutic proteins during manufacturing and storage. These modifications negatively affect efficacy and stability of biotherapeutic proteins. Major non-enzymatic PTMs are deamidation, disulfide bond scrambling and oxidation. These non-enzymatic PTMs are also introduced during protein preparation for peptide mapping and compromise the analysis. In the webinar recording Dr. Saveliev discusses sources of these artificial protein modifications as well as procedural optimizations to suppress these PTMs.

Post-Translational Modification Enzymes: How to Interrogate Key Drug Targets

Cell-Based Assays

Tuesday, April 11, 2017

Hicham Zegzouti, PhD

Post-translational modifications (PTMs), the addition of functional groups to proteins, play a significant role in regulating cellular biology. Examples include phosphorylation, a common mechanism in regulating enzymatic activity, and the addition of carbohydrates to many eukaryotic proteins, which promotes proper folding, improves stability and serves in regulatory functions. A key objective of research and drug discovery surrounding PTMs is to study the regulation of these enzymes and find specific modulators of their activity. Although mass spectrometry, eastern and western blotting detection technologies have been used traditionally, none are amenable to high throughput or as sensitive as bioluminescent detection of nucleotide-based substrates for such enzymes.

Optimizing and Qualifying Reporter Gene Bioassays for Immune Checkpoint Receptors

Drug Discovery

Tuesday, March 28, 2017

Zhijie Jey Cheng, PhD

Reporter gene assays for functional screening and development of antibody drug candidates are gaining popularity due to the significant advantages they offer over traditional methods that rely on human primary immune cells. We will discuss development of a series of MoA-based reporter gene bioassays for antibodies targeting co-inhibitory receptors, co-stimulatory receptors and combination bioassays that target immune checkpoint receptors.

Factors to Consider for Designing and Optimizing Assays Applied to 3D Cultures

Cell-Based Assays

Tuesday, March 14, 2017

Terry Riss, PhD

3D cell culture models present a challenge for using assay chemistries and imaging methods that were originally designed for measuring events from 2D monolayers of cells. In this webinar, we will present factors to consider when designing and optimizing assays applied to 3D cell culture models, case studies describing design of reagents to overcome the limitations inherent with detecting markers from large 3D culture models, and applications of novel real-time detection methods.

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