We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

HaloCHIP™ System Technical Manual

Instructions for Use of Product(s)
G9410
Literature # TM075

The HaloCHIP™ System is a novel system that covalently captures crosslinked protein:DNA complexes from cells without the need for antibodies, providing a robust and efficient alternative to the standard chromatin immunoprecipitation (ChIP) method. The HaloCHIP™ System takes advantage of the HaloTag® protein, which can be fused to any protein and mediates a covalent interaction with various ligands, including a resin-based ligand, HaloLink™ Resin. For this application, a DNA-binding protein of interest is fused to the HaloTag® protein via cloning in a HaloTag® Vector. The recombinant vector construct is transfected into cells for either stable or transient expression. Then the cells are treated with formaldehyde to induce covalent protein:DNA crosslinks, lysed and sonicated to shear the chromatin into smaller fragments for purification by the HaloCHIP™ System. Using the HaloCHIP™ System, these crosslinked complexes can be directly captured from the lysate through covalent binding between the HaloTag® moiety of the fusion protein and the HaloLink™ Resin. Stringent washing removes nonspecific proteins and DNA. Subsequent heating of the HaloTag® fusion protein:DNA complexes on the resin reverses the crosslinks between the DNA and the fusion protein and releases the captured DNA fragments.

Summary of Changes, 12/15:

The following change was made to the 12/15 version of this technical manual: Patent statements were updated.

Printed in USA. Revised 12/15.

Download video:

Closed format: MP4
Open format: OGG
Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom