The MagneSil® Total RNA mini-Isolation System provides a high-throughput 96-well format for fast, simple preparation of intact total RNA from small amounts of cell culture (≤1 × 105 tissue culture cells), tissue (≤2mg tissue lysate in 100μl) or freshly isolated whole blood (≤20μl). The protocol enables high-throughput automated purification on a variety of liquid-handling workstations. Isolation of total RNA in a 384-well format from cell culture (≤1 × 103 cells) and freshly isolated whole blood (≤5μl) also may be performed. Total RNA purification is achieved without vacuum filtration, centrifugation or precipitation. The 96-well procedure takes about 30 minutes to complete using a liquid-handling workstation.
Total RNA purified using this system is suitable for a variety of molecular biology applications including endpoint RT-PCR amplification and real-time RT-PCR.
- Only 30 minutes are required to process one 96-well plate, or 50 minutes for one 384-well plate on a Beckman Coulter Biomek® FX liquid handler.
- Elution volumes as low as 15μl provide concentrated RNA without the need for time-consuming vacuum concentration.
- DNase I treatment is included to remove genomic DNA contamination.
- Validated automated methods are available at: www.promega.com/automethods/