We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

HaloTag® Protein Purification System

G6280

Enhanced Expression and Solubility of Recombinant Proteins with HaloTag® Ligands

  • Covalent, efficient and specific capture of your protein of interest
  • Overcome limitations (i.e., poor capture of proteins expressed at low levels, protein loss during washing) associated with affinity tags
  • Sample size is available (choose Cat.# G6270)

Size

Catalog number selected: G6280

$ 679.00 Your price: Log in

HaloTag® Protein Purification System
1 each
$ 679.00
Your price: Log in
Change Configuration

HaloTag® Protein Purification System

HaloTag® Protein Purification System is designed to purify proteins fused to the HaloTag® novel protein tag that enhances the expression and solubility of recombinant proteins. HaloTag® Technology enables the covalent, efficient and specific capture of a protein of interest onto HaloLink™ Resin, thus overcoming the equilibrium-based limitations associated with affinity tags (i.e., poor capture of proteins expressed at low levels and protein loss during washing of the purification resin.

The HaloTag® technology offers a quick and convenient way to test protein expression of HaloTag® fusion proteins as well as to monitor the efficiency of immobilization to HaloLink™ Resin by labeling with fluorescent HaloTag® TMR Ligand followed by SDS-PAGE analysis. Guidelines can be found in the HaloLink™ Resin Technical Manual #TM250, the HaloLink™ Protein Array Technical Manual #TM310 and the HaloCHIP™ System Technical Manual #TM075.

Use the HaloTag® Protein Purification System for:

  • Purification of poorly expressed fusion proteins
  • Improved yield, purity and specific activity of soluble, functional proteins compared to His-Tag, GST and MBP affinity tags
  • Enhance target protein expression in prokaryotic, mammalian and cell-free systems
  • Recover tag-free target protein using TEV Protease cleavage
  • In-gel detection and quantification of protein expression levels with fluorescent HaloTag® Ligands


Schematic diagram of protein purification using HaloTag Technology.

Schematic of protein purification with HaloTag® fusion tagging.

Specifications

You are viewing: G6280 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

TEV Protease for use with HisLink™ Resin

V109B 1 × 1.6ml

HisLink™ Protein Purification Resin

V882C 1 × 5ml View Product

HaloLink™ Resin

G1915 1 × 25ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,112,552 and 7,354,750.

U.S. Pat. Nos. 7,429,472, 7,888,086, 8,202,700 and other patents pending.

Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

Specifications

You are viewing: G6270 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

TEV Protease for use with HisLink™ Resin

V109A 1 × 200μl

HisLink™ Protein Purification Resin

V882D 1 × 0.5ml View Product

HaloLink™ Resin

G1913 1 × 2.5ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,112,552 and 7,354,750.

U.S. Pat. Nos. 7,429,472, 7,888,086, 8,202,700 and other patents pending.

Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

workflow-background
Choose your country

Americas

Brazil
Canada
United States

Pacific Asia

Australia
India
Japan
Korea, Republic of
Singapore

Europe

Austria
Belgium
Denmark
Estonia
Finland
France
Germany
Iceland
Italy
Luxembourg
Netherlands
Norway
Poland
Spain
Sweden
Switzerland
United Kingdom